A hypoglycemic treatment method for jujube extract

A treatment method and technology for extracting liquid, which is applied in the field of blood sugar-lowering treatment for jujube extracts, and can solve problems such as high sugar content and decreased cAMP content

Active Publication Date: 2021-03-26
CANGZHOU ENJI BIOLOGICAL PROD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to aim at the technical defects of the prior art, and to provide a method for reducing sugar in jujube extracts, so as to solve the technical problem of high sugar content in the jujube extracts in the prior art
[0006] Another technical problem to be solved by the present invention is that the cAMP content in the jujube extract will drop significantly during the process of reducing blood sugar.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A hypoglycemic treatment method for jujube extracts, comprising the following steps:

[0024] (1) Strain activation: Streak the Candida utilis glycerol tube stored at -80°C on the YPD solid medium, culture at 29°C for 24 hours to obtain a single colony, and insert the single colony into 5ml YPD liquid medium Cultivate at 29°C and 200rmp for 24 hours to activate the bacterial liquid, and then transfer the bacterial liquid to 300ml YPD liquid medium with a 2% inoculum size for amplified culture of the strain;

[0025] (2) Dilute and sterilize: Take the jujube extract product produced by Cangzhou Enji Biological Products Co., Ltd., dilute it 5 times with water, heat it to 70°C for 30 minutes, and then cool it for later use;

[0026] (3) Fermentation: insert the bacterium liquid after step (1) activation and amplification into step (2) with an inoculum size of 8% by mass fraction, and ferment jujube juice at 30° C. for 19 hours;

[0027] (4) Sterilization and concentration...

Embodiment 2

[0031] A hypoglycemic treatment method for jujube extracts, comprising the following steps:

[0032] 1) Take the jujube extract, dilute it until the total sugar concentration is 200g / L, then heat it to 68°C for 28min, and cool it to room temperature;

[0033] 2) at 0.5×10 9 Insert the Candida utilis bacterium liquid into the product obtained in step 1) at the inoculum amount of each / mL, and ferment for 12 hours at 28° C.;

[0034] 3) Take the fermented liquid obtained in step 2), heat it to 68°C for 28 minutes, and then concentrate the fermented liquid at the same dilution ratio as in step 1);

[0035] 4) Take the product obtained in step 3), add DPE epimerase at a ratio of fructose content to DPE epimerase mass ratio of 180:1, react at 50°C and pH 5.5 for 2 hours, and then heat Keep at 68°C for 28min.

[0036] On the basis of the above technical solutions, the following conditions are met:

[0037] The method for concentrating the fermented liquid in step 3) is distillati...

Embodiment 3

[0041] A hypoglycemic treatment method for jujube extracts, comprising the following steps:

[0042] 1) Take the jujube extract, dilute it until the total sugar concentration is 300g / L, then heat it to 72°C for 32min, and cool it to room temperature;

[0043] 2) with 10 9 Insert the Candida utilis bacterium liquid into the product obtained in step 1) at the inoculum amount of each / mL, and ferment for 20 h at 32° C.;

[0044] 3) Take the fermented liquid obtained in step 2), heat it to 72° C. for 32 minutes, and then concentrate the fermented liquid at the same dilution ratio as in step 1);

[0045]4) Take the product obtained in step 3), add DPE epimerase at a ratio of fructose content to DPE epimerase mass ratio of 1800:1, react at 60°C and pH 6.5 for 8 hours, and then heat Keep at 72°C for 32min.

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PUM

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Abstract

The invention provides a sugar-reducing treatment method for a jujube extracting solution. According to the sugar-reducing treatment method, in accordance with the nutrient components of the jujube extracting solution and the metabolic characteristic of bacterial strains, candida utilis is selected as a fermentation bacterial strain. Because of the characteristics that the candida utilis is low in requirement for growth factors, and can realize selective metabolism of glucose, cane sugar and raffinose, maltose, galactose, lactose and melibiose are not fermented, besides, the fat and the like are not decomposed, and based on reservation of most of nutrient components of the jujube extracting solution, sugar can be degrated in a pointed manner. Based on this, in combination with a biocatalysis technique, DPE epimerase is used for treating the jujube extraction solution, and conversion of the glucose and levulose into low-calorie low-glycemic-index functional rare sugar is facilitated. According to the method, the glucose content in the jujube extracting solution can be reduced by nearly 70%, the levulose content is reduced by more than 40%, and before and after treatment, the cAMP content is hardly reduced. Obtained products also reserve original flavor characteristics of the jujube extracting solution.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a method for reducing blood sugar of jujube extracts. Background technique [0002] Jujube, as an important edible fruit, has a long history of cultivation in my country. Jujube not only has sweet and mellow taste characteristics, but also has rich nutrients. Take Cangzhou golden silk jujube as an example. Its sugar content is as high as 65%. Every 100 grams of fresh jujube contains 300-600 mg of vitamin C. It is also rich in protein, fat, crude fiber, phosphorus, calcium, iron, potassium, and sodium. , magnesium, chlorine, iodine, niacin and vitamins A and B 1 and other nutrients. In addition, the medicinal effects of jujubes have been recognized by the motherland's medicine since ancient times. Clinically, it is suitable for the treatment of spleen and stomach weakness, deficiency of Qi, anemia and chlorosis, cough due to lung deficiency, insomnia, fatigue, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L19/00A23L5/20
Inventor 刘国洪刘晨田晓丽
Owner CANGZHOU ENJI BIOLOGICAL PROD
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