Purification method for decoloring and deproteinizing Scholar bean polysaccharide

A purification method and deproteinization technology, which is applied in the field of purification of champion bean polysaccharide decolorization and deproteinization, can solve the problems of large amount of reagents, multiple repetitions, high inactivation temperature, etc., and achieve simple and time-saving operation, avoid organic solvents, and process safety Effect

Inactive Publication Date: 2017-06-23
LONGYAN UNIV
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Problems solved by technology

Among them, the organic reagent method has problems such as many repetitions and a large amount of reagents, the enzymatic method has problems such as high cost, and the high inactivation temperature easily destroys the

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  • Purification method for decoloring and deproteinizing Scholar bean polysaccharide
  • Purification method for decoloring and deproteinizing Scholar bean polysaccharide

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Embodiment Construction

[0021] The present invention takes the decolorization rate, protein removal rate and polysaccharide retention rate as comprehensive evaluation indicators, and introduces a comprehensive effect index to objectively evaluate the adsorption performance of 10 kinds of macroporous resins on the crude polysaccharide protein and pigment of the champion bean, and screen out the resin with the best effect , On this basis, the decolorization and deproteinization process conditions of D113 macroporous resin were analyzed and optimized through single factor test and Design-Expert software, and the regeneration conditions of D113 resin were investigated.

[0022] Concrete steps of the present invention are:

[0023] 1. Extraction of champion bean polysaccharide

[0024] Dry the champion beans at 105°C to constant weight, crush them through a 40-mesh sieve, and reflux them in a Soxhlet extractor for 4 hours to remove esters, take them out, and ventilate and dry. The reflux extraction solve...

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Abstract

The invention discloses a purification method for decoloring and deproteinizing Scholar bean polysaccharide. The method comprises the following steps: extracting Scholar beans to obtain Scholar bean crude polysaccharide; performing pretreatment by D113 weakly acidic cation exchange resin, repeatedly rinsing with deionized water until water is clear and not brown, selecting a glass chromatography column, packing the column by a wet method, washing with alkali and deionized water until the pH value is 7, washing with acid and deionized water until the pH value is 7, and soaking with deionized water for future use; adopting D113 weakly acidic cation exchange resin, adding Scholar bean crude polysaccharide with the mass concentration of 4.0 to 4.5 mg/mL, the pH value of 5.3 to 5.8 and the BV volume of 2.7 to 3.2, performing operation at a flow rate of 1.4 to 1.9 mL/min, deproteinizing, decoloring and retaining polysaccharide. The method is simple in operating step, little in influence on the polysaccharide structure, low in cost, environmentally-friendly, rapid in adsorption and high in capacity, and the resin can be reused.

Description

technical field [0001] The invention relates to a purification method for the decolorization and deproteinization of the champion bean polysaccharide. Background technique [0002] At present, the decolorization and deproteinization methods of polysaccharides mainly include: enzymatic method, organic reagent method (Sevage method, trichloroacetic acid method, etc.), adsorption method (activated carbon method, macroporous resin method, etc.), hydrogen peroxide oxidation method, etc. Among them, the organic reagent method has problems such as many repetitions and large amount of reagents, the enzymatic method has problems such as high cost, and high inactivation temperature easily destroys the structure of polysaccharides and destroys the activity, hydrogen peroxide easily causes polysaccharides to degrade and other problems, and activated carbon has problems such as decolorization Long time, large loss of polysaccharides and other problems. The macroporous resin method mainl...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 黎英林标声陈雪梅陈小红
Owner LONGYAN UNIV
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