A kind of aseptic seeding and tissue culture method of micro-spotted primula moss
A technology of primrose and aseptic sowing, applied in the field of bioengineering, can solve problems such as lack of artificial breeding technology, and achieve the effects of high seed germination rate, fast seedling formation and good seedling quality
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Embodiment 1
[0027] A kind of aseptic seeding and tissue culture method of micro-spotted primula moss, comprising the steps:
[0028] (1) Aseptic germination of seeds: take the fruit pods of the mature and uncracked Primula spp., rinse them under running water for 10 minutes with detergent, and use 75% alcohol on the ultra-clean workbench. Soak the fruit pods in medium for 30 seconds, rinse them with sterile water for 3 times, then soak the fruit pods with 0.1% mercuric chloride solution for 12 minutes, rinse them with sterile water for 3 times, cut the fruit pods on the inoculation tray, and gently clean them with a sterilized spoon. The seeds are put into the seed germination medium, and the sown seeds are cultivated in the dark in the light incubator until the seeds germinate, and then transferred to the light culture in the culture room; the temperature of the dark culture is 17°C; The light culture temperature is 23°C, and the light is 1000lx; the germination medium is MS+GA 3 1.0mg...
Embodiment 2
[0035] A kind of aseptic seeding and tissue culture method of micro-spotted primula moss, comprising the steps:
[0036](1) Aseptic germination of seeds: take the fruit pods of the mature and uncracked Primula spp., rinse them under running water for 15 minutes after soaking them with detergent, and use 75% alcohol on the ultra-clean workbench. Soak the fruit pods in medium for 40 seconds, rinse them with sterile water for 5 times, then soak the fruit pods with 0.1% mercuric solution for 15 minutes, rinse them with sterile water for 5 times, cut the fruit pods on the inoculation tray, and gently clean them with a sterilized spoon. Dial the seeds into the seed germination medium, and cultivate the sown seeds darkly in the light incubator until the seeds germinate, and then transfer them to the light culture in the culture room; the temperature of the dark culture is 20°C; The light culture temperature is 25°C, and the light is 1400lx; the germination medium is MS+GA 3 2.0mg / L...
Embodiment 3
[0043] A kind of aseptic seeding and tissue culture method of micro-spotted primula moss, comprising the steps:
[0044] (1) Aseptic germination of seeds: take the fruit pods of the mature and uncracked Primula spp., rinse them under running water for 13 minutes after soaking them with detergent, and use 75% alcohol on the ultra-clean workbench. Soak the fruit pods in medium for 35 seconds, rinse them with sterile water for 3-5 times, then soak the fruit pods with 0.1% mercury liter solution for 14 minutes, rinse them with sterile water for 4 times, cut the fruit pods on the inoculation tray, and use a sterilized spoon Gently dial the seeds into the seed germination medium, and cultivate the sown seeds darkly in the light incubator until the seeds germinate, and then transfer them to the light culture in the culture room; the temperature of the dark culture is 18°C; The above light culture temperature is 24°C, and the light is 1200lx; the germination medium is MS+GA 3 1.5mg / ...
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