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Gynogenesis induction method of penaeus monodon

A technology for gynogenesis and penaeid monodon is applied in the field of gynogenesis induction of penaeus monodon, and the method is simple and effective.

Inactive Publication Date: 2017-06-30
QINGDAO QINGQUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, this method has not been reported in the study of gynogenesis in marine organisms.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] The present invention will be described in further detail below in conjunction with specific embodiments.

[0015] Rearing of parents and collection of sperm and eggs

[0016] Monodon prawns were temporarily raised in circulating seawater at 14°C after being retrieved, and the male and female individuals were temporarily raised separately. Feed the bait. During the temporary raising period, the temperature is increased by 1°C every day, and the temperature is kept constant at 16-17°C for production. The eggs were obtained by light stimulation method. After the eggs were produced, the eggs were first filtered through a 300-mesh sieve, and then collected by a 500-mesh sieve. After dilution, the density of the quantitative eggs was observed under a microscope. Adjust the density of eggs according to the experimental needs.

[0017] Electrostimulation Treatment of Eggs of Penaeus monodon

[0018] Induction of gynogenesis of eggs: Electric stimulation method is used. Bef...

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Abstract

The invention relates to a gynogenesis induction method of penaeus monodon, an electrostimulation method is adopted, before electrostimulation, eggs are shifted into an electrostimulation buffer solution to be balanced by 2 min, the parameters of an electric impulser are adjusted, 1 ml of eggs are absorbed with a suction tube and placed into an electric shock cup, and then electrostimulation treatment can be performed; the electrostimulation buffer solution is a sorbitol buffer solution which is specifically prepared from 0.25 mmol / L of Mg(C2H3O2)2.4H2O, 0.5 mol / L of Hepes, 0.1 mmol / L of Ca(C2H3O2)2H2O, 1% of BSA and 0.25 mmol / L of sorbitol. The parameters of the electric impulser are that the field intensity is 1.75 kv / cm, the capacitance is 3 mu F, and the electric pulse is performed by 3 times. The egg density during the electrostimulation is 11.25*104 pieces / ml. The method is simple and efficient, the cleavage rate of the penaeus monodon can reach 28% or above after electrostimulation, and the embryo survival rate can reach 98% or above.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a method for inducing gynogenesis of Penaeus monodon. Background technique [0002] Artificial induction of gynogenesis is conducive to the rapid establishment of pure lines, so it is of great significance to the research of genetic breeding and basic biology. In terms of marine economic shellfish, research in this field is relatively lagging behind. Currently, only about ten species of marine economic shellfish have been artificially induced to study gynogenesis. These studies mainly use various radiation or chemical methods to inactivate sperm, thereby inducing gynogenesis of eggs. The homologous sperm inactivation method commonly used in research is difficult to eliminate the interference of exogenous sperm genome, so in recent years, some scholars have tried to use heterologous sperm induction to eliminate this interference, such as studies in clams such...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/59
CPCY02A40/81
Inventor 褚方强
Owner QINGDAO QINGQUAN BIOTECH CO LTD