Method for preparation of heparan sulfate from crude sheep heparin sodium extraction wastewater

A technology of heparan sulfate and heparin sodium, applied in the field of biochemical industry, can solve the problems of unextracted, waste of resources, etc., and achieve the effects of realizing reuse, stable product quality and simple operation.

Inactive Publication Date: 2017-06-30
山东绅联药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is a large amount of heparan sulfate in the extraction waste liquid of crude heparin sodium, which is discharged during the production process without being extracted, resulting in a waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for preparing heparan sulfate from waste liquid extracted from heparin sodium crude product, adopts the following steps:

[0023] (1) Dilution: Add 1300L of water to 2000L of the crude heparin sodium extraction waste liquid to dilute the waste liquid;

[0024] (2) Resin adsorption: Add 13kg of resin, control the temperature at 55-60°C, pH 7.5, and stir for 9 hours.

[0025] (3) Washing: Drain the adsorption waste liquid, collect the resin, add 19.5kg of purified water, stir for 30 minutes, drain the washing water; add 19.5L of 0.4mol / L sodium chloride solution, stir the resin for 30 minutes, drain and wash liquid;

[0026] (4) Elution: Add 19.5L of 2.1mol / L sodium chloride solution, maintain the temperature at 45-60°C, pH7.0, stir and elute for 2 hours, and collect the eluate;

[0027] (5) Precipitation: add 1.2 times of ethanol for elution, stir for 30 minutes and let it stand for 8 hours to collect the heparan sulfate precipitate.

[0028] (6) Drying: add ...

Embodiment 2

[0030] A method for preparing heparan sulfate from waste liquid extracted from heparin sodium crude product, adopts the following steps:

[0031] (1) Dilution: Add 1400L of water to 1800L of the crude heparin sodium extraction waste liquid to dilute the waste liquid;

[0032] (2) Resin adsorption: Add 12kg of resin, control the temperature at 55-60°C, pH8.0, and stir for 9 hours.

[0033] (3) Washing: drain the adsorption waste liquid, collect the resin, add 18kg of purified water, stir for 30 minutes, drain the washing water; add 18L of 0.4mol / L sodium chloride solution, stir the resin for 30 minutes, and drain the washing liquid;

[0034] (4) Elution: add 18L of 2.1mol / L sodium chloride solution, maintain the temperature at 45-60°C, pH8.5, stir and elute for 2 hours, and collect the eluate;

[0035] (5) Precipitation: add 1.1 times ethanol for elution, stir for 30 minutes and then let stand for 8 hours to collect the heparan sulfate precipitate.

[0036] (6) Drying: add th...

Embodiment 3

[0038] A method for preparing heparan sulfate from waste liquid extracted from heparin sodium crude product, adopts the following steps:

[0039] (1) Dilution: Add 1300L of water to 1500L of the crude heparin sodium extraction waste liquid to dilute the waste liquid;

[0040] (2) Resin adsorption: Add 10kg of resin, control the temperature at 55-60°C, pH 7.8, and stir for 10 hours.

[0041] (3) Washing: drain the adsorption waste liquid, collect the resin, add 15kg of purified water, stir for 30 minutes, drain the washing water; add 15L of 0.4mol / L sodium chloride solution, stir the resin for 30 minutes, and drain the washing liquid;

[0042] (4) Elution: add 15L of 2.1mol / L sodium chloride solution, maintain the temperature at 45-60°C, pH7.5, stir and elute for 2 hours, and collect the eluate;

[0043] (5) Precipitation: add 1.2 times of ethanol for elution, stir for 30 minutes and let it stand for 8 hours to collect the heparan sulfate precipitate.

[0044] (6) Drying: add...

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Abstract

The invention belongs to the field of biochemical industry, and in particular relates to a method for preparation of heparan sulfate from crude sheep heparin sodium extraction wastewater. The heparan sulfate is prepared by dilution, adsorption, washing, elution, precipitation and drying of the crude sheep heparin sodium extraction wastewater, and the method comprises (1) dilution, (2) adsorption, (3) washing, (4) elution, (5) precipitation and (6) drying. The method overcomes the problems that the heparan sulfate only can be extracted from a refined heparin sodium product, realizes the reuse of waste resources, has the advantages of simple operation and stable product quality, and is suitable for large-scale production.

Description

[0001] (1) Technical field [0002] The invention belongs to the field of biochemical industry, in particular to a method for preparing heparan sulfate from waste liquid extracted from crude heparin sodium. [0003] (2) Background technology [0004] Heparan sulfate is a natural glycosaminoglycan, similar in structure to heparin sodium, but has a smaller molecular weight, higher degree of acetylation, less sulfate group content, and moderate anticoagulant activity. Heparan sulfate is used for antithrombotic, anti-inflammatory, hypolipidemic, and renal protection of diabetic patients. It is the most popular mucopolysaccharide biochemical drug studied this year. [0005] The traditional process extracts from the by-product of heparin sodium boutique, and the extraction amount is small. A large amount of heparan sulfate is contained in the waste liquid extracted from crude heparin sodium, which is discharged during the production process but not extracted, resulting in a waste of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
CPCC08B37/0075
Inventor 谢成君刘敏甄爱华刘建洋
Owner 山东绅联药业股份有限公司
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