Data acquisition method of non-collinear optical parametric amplification fluorescence spectrophotometer
A technology of optical parametric amplification and fluorescence spectrometer, which is applied in the field of data acquisition of non-collinear optical parametric amplification fluorescence spectrometer, which can solve the problems of long data collection time, inability to effectively eliminate noise, and difficulty in ensuring samples
Active Publication Date: 2017-06-30
HEBEI UNIVERSITY
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[0006] The purpose of the present invention is to provide a data acquisition method of a non-collinear optical parametric amplification fluorescence spectrometer to solve the problems in the prior art that the noise cannot be effectively eliminated and the data acquisition time is too long to ensure the accuracy of the sample measurement results
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[0060] The non-collinear optical parametric amplification fluorescence spectrometer data collection method of the present invention comprises the following steps:
[0061] 1) The pulsed laser with a central wavelength of 800nm is split into two beams by the optical beam splitter 3-2. The transmitted beam is used to generate the sample excitation light, and the reflection path is used to generate the optical parametric process pump light;
[0062] 2) The 800nm transmitted fundamental frequency light is converted into a 532nm pulse laser by the sample excitation light part 3-3, and then converged by the focusing lens 3-4 to excite the Rhodamine 6G solution placed in the sample cell 3-5. The sample cell is generally placed near the focal point of the excitation light, close to the side of the first focusing lens 3-4, so as to keep the focal point of the excitation light as small as possible, but avoid damage to the sample due to excessive energy density.
[0063] 3) The fluor...
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Abstract
The invention relates to a data acquisition method of a non-collinear optical parametric amplification fluorescence spectrophotometer. In a non-collinear optical parametric amplification process, on a basis that a pump light, a fluorescence path and an idler beam path meet conservation of energy and conservation of momentum, the intensity of the fluorescence path and the intensity of the idler beam path have high correlation; a to-be-detected fluorescence signal and a to-be-detected idler beam signal after energy is amplified are respectively acquired, and a correlation operation is performed on sampling results of the fluorescence path and the idler beam path, so as to obtain a cross-correlation result of a to-be-detected fluorescence and a to-be-detected idler beam when in zero delay; a positive square root of the cross-correlation result represents a signal value of the to-be-detected fluorescence on a kinetic curve when in different time delays. According to the data acquisition method, the noise of a non-collinear optical parametric amplification fluorescence spectrophotometer system can be more effectively eliminated, the SNR (signal to noise ratio) of the obtained kinetic curve of the fluorescence is improved, the fluorescence spectrophotometer with higher time resolution is realized, the measurement time is shortened, and the accuracy of a measurement result of an active sample is ensured.
Description
technical field [0001] The invention relates to an ultrafast time-resolved fluorescence spectrum measurement technology, in particular to a data acquisition method of a non-collinear optical parameter amplification fluorescence spectrometer. Background technique [0002] The time resolution of non-collinear optical parametric amplification fluorescence spectroscopy can reach the order of 10 femtoseconds, which can be used to analyze the processes of energy transfer, electron transfer, and structural changes in the system. The working principle of non-collinear optical parametric amplification fluorescence spectroscopy is as follows: figure 1 (Journal Of The Optical Society Of American B, 2009, 26, 8, 1627). figure 1 Among them, "Signal" means the fluorescence to be measured, and "Pump" means the femtosecond pulsed laser, that is, the pump light. Usually the duration of the fluorescence signal to be measured is much longer than that of the femtosecond pulse laser (Pump). I...
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IPC IPC(8): G01N21/64
CPCG01N21/6408
Inventor 王凯党伟刘东州李文华赵晓辉
Owner HEBEI UNIVERSITY