Reprogramming method for induced pluripotent stem cells

A pluripotent stem cell and reprogramming technology, applied in the field of reprogramming from adult stem cells to induced pluripotent stem cells, can solve the problems of iPSC formation, low immunogenicity, and difficulty in detecting teratomas, and achieve high reprogramming efficiency , low immunogenicity, and the effect of eliminating safety concerns
CN106916850AActive Publication Date: 2017-07-04ZONHON BIOPHARMA INST
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZONHON BIOPHARMA INST
Publication Date
2017-07-04

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Abstract

The invention relates to a reprogramming method for human induced pluripotent stem cells. The reprogramming method is more favorable for preclinical research and mainly employs Sendai virus-mediated infection of the human induced pluripotent stem cells and reprogramming. The reprogramming method provided by the invention has high reprogramming efficiency; and the obtained human induced pluripotent stem cells have the characteristics of low immunogenicity, high security and the like. Moreover, compared with reprogramming methods for lentivirus-mediated human induced pluripotent stem cells, the reprogramming method provided by the invention has the following advantage that the human induced pluripotent stem cells obtained by using the method is free of integration of exogenous genes and thus has higher safety.
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Description

technical field

[0001] The invention relates to the field of cells, in particular to a method for reprogramming adult stem cells into induced pluripotent stem cells (iPSCs). Background technique

[0002] The inner cell mass in the blastocyst stage of the early vertebrate embryo is pluripotent, and it can differentiate into all types of cells in the three germ layers of the body except the placenta. These terminally differentiated cells generally do not change their fate in vivo. Some studies have shown that through nuclear transfer, cell fusion and co-cultivation of pluripotent cell extracts, terminally differentiated cells can be re-differentiated into a pluripotent state (Yamanaka S, Blau HM, 2010, Nuclear reprogramming to a pluripotent state by three approaches. Nature 465:704-712). However, these methods all rely on oocytes as a scarce material, so they are limited in application.

[0003] In 2006, Yamanaka used retroviral vectors to overexpress four exogenous transcri...

Claims

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