Scaffold with copper carboxymethyl chitosan-sodium alginate and preparation method thereof and applications

A technology of carboxymethyl chitosan and sodium alginate, applied in medical science, tissue regeneration, prosthesis, etc., can solve problems such as uneven distribution of copper ions, immediate cross-linking of solutions, and limitations in practical applications, and achieve good antibacterial properties Effect, good biocompatibility, simple preparation process

Inactive Publication Date: 2017-07-07
GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this synthetic method is prone to the phenomenon of immediate solution cross-linking, which leads to difficult preparation and uneven distribution of copper ions, which severely limits its practical application.
Among the currently reported copper-containing bone repair scaffolds, there are few reports using nano-copper synthesis

Method used

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  • Scaffold with copper carboxymethyl chitosan-sodium alginate and preparation method thereof and applications
  • Scaffold with copper carboxymethyl chitosan-sodium alginate and preparation method thereof and applications
  • Scaffold with copper carboxymethyl chitosan-sodium alginate and preparation method thereof and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Prepare 10 mL of nano-copper solution with a concentration of 10 mM in ultrapure water, and stir with a magnetic stirrer at room temperature until uniform. Gradually add 150 mg of carboxymethyl chitosan and 150 mg of sodium alginate, and stir with a magnetic stirrer at room temperature until completely dissolved. The above mixed solution was added to a 48-well culture plate, and placed in a -80°C refrigerator overnight. The 48-well plate was transferred to a lyophilizer for 48 h. Add 2% CaCl 2 The solution was cross-linked for 30 minutes, and washed three times with ultrapure water. Put it into a -80°C refrigerator and freeze it overnight, and use a freeze dryer to freeze-dry for 24 hours to obtain a scaffold, which is a copper-containing carboxymethyl chitosan-sodium alginate scaffold.

[0038] Combine the resulting bracket with 10 5 Fibroblasts L929 were co-cultured, the medium was discarded at 1, 3, and 5 days, and 10% MTT solution was added. After 4 hours, DMSO ...

Embodiment 2

[0044] Prepare 10mL nano-copper solution with a concentration of 1mM in ultrapure water, and stir with a magnetic stirrer at room temperature until uniform. Gradually add 150 mg of carboxymethyl chitosan and 150 mg of sodium alginate, and stir with a magnetic stirrer at room temperature until completely dissolved. The above mixed solution was added to a 48-well culture plate, and placed in a -80°C refrigerator overnight. The 48-well plate was transferred to a lyophilizer for 48 h. Add 2% CaCl 2 The solution was cross-linked for 30 minutes, and washed three times with ultrapure water. Put it into a -80°C refrigerator and freeze it overnight, and use a freeze dryer to freeze-dry for 24 hours to obtain a scaffold, which is a copper-containing carboxymethyl chitosan-sodium alginate scaffold.

[0045] Compared with the blank culture plate, the copper-containing carboxymethyl chitosan-sodium alginate scaffold prepared in this example had relative cell viability of 85.81%, 132.19%...

Embodiment 3

[0049] Prepare 10mL nano-copper solution with a concentration of 0.1mM in ultrapure water, and stir with a magnetic stirrer at room temperature until uniform. Gradually add 150 mg of carboxymethyl chitosan and 150 mg of sodium alginate, and stir with a magnetic stirrer at room temperature until completely dissolved. The above mixed solution was added to a 48-well culture plate, and placed in a -80°C refrigerator overnight. The 48-well plate was transferred to a lyophilizer for 48 h. Add 2% CaCl 2 The solution was cross-linked for 30 minutes, and washed three times with ultrapure water. Put it into a -80°C refrigerator and freeze it overnight, and use a freeze dryer to freeze-dry for 24 hours to obtain a scaffold, which is a copper-containing carboxymethyl chitosan-sodium alginate scaffold.

[0050] Compared with the blank culture plate, the copper-containing carboxymethyl chitosan-sodium alginate scaffold prepared in this example had relative cell viability of 94.97%, 133.8...

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Abstract

The invention discloses a preparation method of a copper-containing carboxymethyl chitosan-sodium alginate support, which comprises the following steps: (1) preparing a nano-copper solution with ultrapure water; (2) preparing the carboxymethyl chitosan and sodium alginate are added to the nano-copper solution prepared in step (1), and mixed uniformly to obtain a mixed solution; (3) freeze-drying the mixed solution obtained in step (2) to obtain a freeze-dried initial scaffold; (4) Adding CaCl2 solution to the initial stent obtained in step (3) for cross-linking, washing to obtain a cross-linked stent; (5) lyophilizing the cross-linked stent obtained in step (4) to obtain the obtained Copper-containing carboxymethyl chitosan-sodium alginate antibacterial scaffold for bone repair. The copper-containing carboxymethyl chitosan-sodium alginate scaffold prepared by the preparation method of the invention has a porous structure and good biocompatibility and antibacterial effect.

Description

technical field [0001] The invention relates to a carboxymethyl chitosan-sodium alginate material, in particular to a preparation method and application of a copper-containing carboxymethyl chitosan-sodium alginate scaffold that can be used as an antibacterial bone repair material. Background technique [0002] Infected bone defect is a thorny clinical problem, and the current common clinical treatment methods are debridement, the use of a large amount of antibiotics and bone grafting. Bone tissue engineering is a research hotspot in bone repair. In antibacterial bone repair materials, most of the current research uses the method of adding antibiotics to achieve antibacterial effect. However, the problem of bacterial resistance produced by antibiotics has also become a new problem in the treatment of infections. Chitosan and sodium alginate are polysaccharides abundant in nature and have good application prospects in bone repair materials. A large number of studies have pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/02A61L27/50A61L27/54A61L27/56
CPCA61L27/20A61L27/025A61L27/50A61L27/54A61L27/56A61L2300/102A61L2300/404A61L2430/02
Inventor 陆遥夏虹尹庆水李丽华王晓岚李梅林泽枫张余
Owner GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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