Hepatitis B recombinant antigen, expression gene of hepatitis B recombinant antigen, construction method of hepatitis B recombinant antigen, virus-like particle of hepatitis B recombinant antigen, preparation method of virus-like particle of hepatitis B recombinant antigen, applications of hepatitis B recombinant antigen, and vaccine

Abstract

The invention relates to a hepatitis B recombinant antigen, an expression gene of the hepatitis B recombinant antigen, a construction method of the hepatitis B recombinant antigen, a virus-like particle of the hepatitis B recombinant antigen, a preparation method of the virus-like particle of the hepatitis B recombinant antigen, applications of the hepatitis B recombinant antigen, and a vaccine. The hepatitis B recombinant antigen is obtained by expressing a polynucleotide comprising a nucleotide sequence shown in the SEQ ID NO.1. The expression gene of the hepatitis B recombinant antigen is obtained by splicing a preS region of the hepatitis B virus with a signal peptide region of an influenza virus hemagglutinin and splicing a transmembrane region of the influenza virus with an intracellular region, the expression gene is successfully expressed to obtain the hepatitis B recombinant antigen, and the hepatitis B recombinant antigen is better in immunogenicity, and can enable the immune system of the minority (about 5%-10%) incapable of producing antibodies for the traditional vaccine to make an immune response. Meanwhile, on the basis of the indispensable importance of the preS region for the hepatitis B virus, the vaccine for broader intended population is prepared for the preS region. The vaccine has the effects of preventing infection of the hepatitis B virus and clearing away the hepatitis B virus, and is expected to be transformed into a novel preventive and therapeutic hepatitis B vaccine on medical science.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a hepatitis B recombinant antigen and its expression gene, a construction method, a virus-like particle and its preparation method, application and vaccine. Background technique [0002] Hepatitis B is one of the major infectious diseases of human beings, affecting as many as 300 million people worldwide. According to the survey, about one tenth of the people in China are carriers of hepatitis B virus. Hepatitis B virus infection can cause acute and chronic hepatitis in humans, and hepatitis can develop into more serious diseases, such as liver cirrhosis and liver cancer. [0003] Hepatitis B Virus (HBV), referred to as HBV, belongs to the hepadnaviridae family and is susceptible to humans and chimpanzees. There are three kinds of antigen molecules on the surface of hepatitis B virus particles, which are the small protein (the small protein, S protein), the middle protein (...

AI Technical Summary

Problems solved by technology

[0005] Based on this, it is necessary to address the problem of poor immunogenicity of traditional hepatitis B virus vaccines, and provide a recombinant hepatitis B antigen with relatively good immunogenicity and its expression gene, construction method, virus-like particle and its preparation Methods, Applications and Vaccines

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