In-vivo gene silencing method taking lentivirus as tool
A technology of gene silencing and lentivirus, which is applied in the fields of molecular genetic biology and developmental biology, can solve the problems of high cost, side effects, and time-consuming, and achieve the effect of simple method
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[0019] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0020] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.
[0021] Such as figure 1 As shown, the in vivo gene silencing method using lentivirus as a tool provided by the embodiment of the present invention includes the following steps:
[0022] S101: Use a 5ul micro-injector to inject the virus, the injection volume is set to 1ul per 20g egg weight, and the virus titer is uniformly 3x10 8 TU / ml;
[0023] S102: 2-day-old chicken embryos, open a hole with a diameter of about 1.5-2 mm in the air cell, visually determine the position of the air...
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