Method for preparing glucose syrup for fermentation through two-enzyme method rice bran starch hydrolysis

Abstract

The invention discloses a method for preparing glucose syrup for fermentation through two-enzyme method rice bran starch hydrolysis. The method comprises the following steps that 1, rice bran is weighed, impurities are removed through washing, then water is added for pulp mixing, a CaC12 solution is added to improve the activity of liquifying enzyme, pH of pulp is regulated to be 5.5-6.4, 0.025%-0.15% of liquifying enzyme is added by the total weight of the pulp, liquification is performed at the temperature of 80-100 DEG C for 60-180 minutes, and the pulp is subjected to suction filtration to obtain a filtered solution, namely a liquified solution; 2, the liquified solution in the step 1 is taken, a pH value is regulated to be 3.0-6.0, 0.01%-0.05% of liquifying enzyme is added by the total weight of the pulp, saccharification is performed at the temperature of 40-80 DEG C for 24-60 hours, and the DX value of the saccharified liquid is sampled and determined after saccharification is completed. According to the method, the rice bran is adopted as a raw material, rice bran starch hydrolysis is performed by adopting a two-enzyme method, gelatinization and liquification are performed by adopting a one-step method, the best process condition for the one-step method for gelatinization and liquification is obtained, and a theoretical basis of production is provided for glucose production based on an industrial rice bran starch enzyme method.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for preparing glucose by hydrolyzing rice bran starch, in particular to a method for preparing glucose syrup for fermentation by hydrolyzing rice bran starch with a double enzyme method. Background technique [0002] Rice bran is a by-product produced during the milling process of rice. It is a mixture of the cortex and a small amount of rice germ and broken rice removed during the milling process of brown rice, accounting for about 5%-8% of the mass of rice. my country's annual output of rice bran is 10 million tons, so rice bran resources are a renewable, cheap and huge amount of low-value biomass raw materials in China. However, most of the rice bran in our country is treated as geothermal fuel or feed, and only 10%-15% of rice bran is used to extract oil or extract high-value products such as calcium phytate, inositol, oryzanol, etc., to produce these high-value product...

AI Technical Summary

Problems solved by technology

In addition, because the acidification solution needs to be carried out under high temperature, high pressure and acidic conditions, the compound reaction and decomposition reaction of glucose will occur in acid liquefaction, which will affect the yield of glucose. The DX value is low, about 90%, and the by-products of acidolysis , increasing the difficulty of refining the saccharification solution

The law of acid hydrolysis cannot be controlled by itself, and it is difficult to directional produce various sugars

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