Cheap micro-fluidic device for diagnosis of cholera and with specific liquid flow transmission manner

Abstract

The invention relates to a cheap micro-fluidic device for diagnosis of cholera and with a specific liquid flow transmission manner, belonging to the field of analysis and testing. A series of obstacles exist in preparation of a substrate for a cholera diagnosis micro-fluidic chip from cheap and easily workable polydimethylsiloxane (PDMS); a PDMS material has a strongly hydrophobic surface and the effect of targeted surface modification cannot last for a long time; and the invention aims to overcome a series of such problem. According to main points of a technical scheme in the invention, PDMS with an original surface is selected as a substrate; a minisized ultrasonic transducer is arranged at a position close to a sample liquid flow terminal of a micro-fluidic chip in an adhesion manner, and an ultrasonic wave reducing device is arranged on the sample introduction terminal of the micro-fluidic chip, so the intensity of ultrasonic waves rapidly and progressively decreases in a short distance; thus, difference of interfacial tension at the two terminals of the chip is formed, which leads to formation of pressure difference between the two terminals, and the pressure difference drives sample liquid flow to flow towards the sample liquid flow terminal along a capillary channel.

Description

technical field [0001] The invention relates to a low-cost microfluidic device for cholera diagnosis with a specific liquid flow transmission mode, which belongs to the field of analysis and testing. Background technique [0002] For the technical background of multi-channel microfluidic cholera diagnosis, please refer to CN 200910150930.4 and other invention patent applications. [0003] As far as the overall overview of microfluidic technology itself is concerned, you can refer to the monograph "Illustrated Microfluidic Chip Laboratory" published by the famous microfluidic expert Mr. Lin Bingcheng not long ago, which has been published by Science Press. The past, present, and future prospects of microfluidic technology, etc., have detailed and long-form discussions that go deep into specific details. [0004] So, let's talk about the key issues of this case. [0005] The basic structure of a microfluidic chip includes a substrate etched with tiny liquid flow channels and...

AI Technical Summary

Problems solved by technology

[0007] But it's not that simple

[0008] First, this polydimethylsiloxane material, the material referred to by the acronym PDMS, is itself a strongly hydrophobic material. Microchannels are built on this material. If the microchannels are not targeted The modification operation of the surface of the channel, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the micro channel in the structure occupies most of the inner surface of the liquid flow channel, then the PDMS micro channel The strong hydrophobic characteristic of the inner surface of the channel is the decisive factor, which will make it very difficult for the polar liquid flow similar to the aqueous solution to pass through, and its flow resistance is so large that even ordinary micropumps are difficult to push. Of course, If the cover sheet also chooses to use the PDMS material, then the problem is basically the same, with little difference; therefore, in the prior art, it is necessary to modify and modify the inner surface of the microchannel on the PDMS material; then , is this modification operation for the inner surface of the PDMS microchannel very troublesome? That's not the problem. What constitutes a serious technical problem i

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