A kind of multiple PCR kit and detection method for transgenic soybean detection

A genetically modified soybean and kit technology, which is applied in biochemical equipment and methods, recombinant DNA technology, and microbial determination/inspection, etc., can solve the problem that inspection results are prone to false negatives, the detection technology cannot be effectively promoted and applied, and the method has poor repeatability, etc. problem, to achieve the effect of saving reagent usage, reducing detection cost, and simple operation
CN106995841BActive Publication Date: 2020-10-09SUZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SUZHOU UNIV
Publication Date
2020-10-09

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Abstract

The invention discloses a multiplex PCR kit and detection method for transgenic soybean detection, which can quickly detect 12 kinds of transgenic soybeans approved for import by the Ministry of Agriculture of my country, including MON87701, GTS40-3-2, MON89788, CV127, A2704, A5547, DP356043 , DP305423, MON87769, MON87708, 305423×40‑3‑2, MON87701×MON89788; the multiplex PCR kit disclosed by the present invention is based on the multiplex PCR method mediated by “public primers”, including public primers, multiplex primers, and multiplex PCR system detection The two-step annealing temperature method improves the specificity and sensitivity of the multiplex PCR system. The multiplex PCR method is inclusive and can continue to increase detection targets, which is conducive to improving the detection throughput of multiplex PCR. The invention has the advantages of high sensitivity, economy, speed, simple operation and the like, and can be applied to food detection.
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Description

technical field

[0001] The invention belongs to the field of food / gene detection, and in particular relates to a multiple PCR kit and a detection method for transgenic soybean detection. Background technique

[0002] At present, the detection technologies of genetically modified crops reported at home and abroad are mainly divided into three categories, one is nucleic acid-based detection technology; the other is protein-based enzymatic and immunological detection technology; Biosensors and biochips for technological development, near-infrared spectroscopy and mass spectrometry analysis techniques based on modern analytical instruments, etc. The second type of protein-based detection technology is to detect proteins expressed by exogenous genes in transgenic plants. These proteins are easily damaged and inactivated, decomposed or disappeared during processing. The results are prone to false negatives and the method has poor repeatability. New technologies for genetically mo...

Claims

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