Integrated electrochemical light emitting paper micro-fluidic chip and preparation method and application thereof

Abstract

The invention discloses an integrated electrochemical light emitting paper micro-fluidic chip, a preparation method and a method using the chip to detect hepatitis B surface antigen. The preparation method of the paper micro-fluidic chip comprises the following steps of preparing an electrode layer; preparing a paper channel layer on paper, immobilizing antibodies in an antibody embedding area and a detection area, and covering the surface of a sample area with a blood filtering film, so as to functionalize the paper channel layer; using a hot press technique to prepare the electrochemical light emitting paper micro-fluidic chip which comprises an upper sealing layer, the paper channel layer, the electrode layer and a lower sealing layer from top to bottom. The method using the chip to detect the hepatitis B surface antigen comprises the following steps of dripping a full blood sample into a sample adding area, enabling the sample to generate immune reaction in the flowing process, cleaning, and putting into an electrochemical light emitting detector for detection. The integrated electrochemical light emitting paper micro-fluidic chip has the advantages that the functions are rich, the detection sensitivity is high, the use is simple and convenient, and the cost is low; the sample pretreatment, mixing, reaction and detection are integrated, and the chip can be widely applied to the field of medical detection.

Description

technical field [0001] The invention belongs to the field of medical detection, and relates to an integrated electrochemiluminescence paper microfluidic chip and a preparation method of the chip, and also relates to a method for detecting hepatitis B surface antigen by using the chip. Background technique [0002] The detection of hepatitis B surface antigen (HBsAg) is an important indicator for the clinical diagnosis of hepatitis B. At present, enzyme-linked immunoassay and Roche electrochemiluminescence detection system are commonly used to detect it. Enzyme-linked immunoassay is cumbersome to operate and cannot perform quantitative detection, so it is not suitable for real-time rapid detection; while Roche's electrochemiluminescence detection system has the limitations of bulky equipment and high price, and is not suitable for on-site instant detection and widespread use. Therefore, there is an urgent need for a new detection method with high detection sensitivity, simple...

AI Technical Summary

Problems solved by technology

Yu Jinghua's research group combined electrochemiluminescence detection with paper microfluidic chips for the first time, and prepared a three-dimensional paper chip based on electrochemiluminescence detection, which realized the detection of tumor markers, but there are still some limitations in its practical application However, the structure of the paper channel of the paper chip is relatively simple, and steps such as sample separation, reagent dripping, washing and separation are still required manually, and the operation is cumbersome and time-consuming, which is not conducive to wide application.

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