Method for identifying cashmere and fine wool
A technology for cashmere and wool, which is applied in the field of textile testing, can solve the problems that cashmere and fine wool are difficult to distinguish, and achieve stable testing results, good stability, and outstanding promotion prospects
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Embodiment 1
[0045] Embodiment 1 (the specificity experiment of cashmere, fine wool composition identification method)
[0046] 1. Materials
[0047] Standard cashmere fiber samples, standard fine wool fiber samples, and standard rabbit cashmere fiber samples are provided by Tianjin Textile Engineering Research Institute.
[0048] 2. Method
[0049] Take the cashmere fiber standard product and the fine wool fiber standard product respectively and mix them. The above-mentioned mixed samples, standard cashmere fiber samples, standard fine wool fiber samples, and standard rabbit cashmere fiber samples were used as test materials, the total protein was extracted, and biomass spectrometry was used to detect cashmere signature peptides YAVPVVTVSSPE, CGPCSSYVR and fine wool landmarks. Peptides TCCEPTVCQSTCYQPTPCVSSPVR, SHSAWSILPR.
[0050] (1) Protein extraction from textile fiber samples:
[0051] The textile fiber samples were shredded, soaked in ethanol, and soaked in chloroform-methanol s...
Embodiment 2
[0059] Example 2 (establishment of cashmere component marker peptide quantitative standard curve)
[0060] 1. Materials
[0061] Cashmere fiber standard products, fine wool fiber standard samples.
[0062] 2. Method
[0063] Take standard cashmere samples and mix them with fine wool fiber standard samples in different proportions to obtain a series of standard samples with cashmere content of 5%, 10%, 25%, 50%, 75%, and 100%, and extract the total protein , add an equal amount of bovine serum albumin (BSA), and use the bovine serum albumin peptide LVNELTEFAK as an internal standard to detect the mass spectrometry signal of the cashmere signature peptide YAVPVVTVSSPE relative to the internal standard peptide, and mix the samples according to different ratios. Different mass spectrometry signals of peptides were used to establish a standard curve for actual sample quantification.
[0064] 3. Results
[0065] like Figure 5 As shown in Table 1, based on the analysis of the c...
Embodiment 3
[0068] Embodiment 3 (identification analysis of cashmere, fine wool in test material)
[0069] 1. Materials
[0070] Commercially available cashmere fabrics S1 and S2. The proportion of cashmere in S1 and S2 is about 70-85% through the fiber fineness tester
[0071] 2. Method
[0072] Treat each test product separately, extract the total protein, add an equal amount of bovine serum albumin (BSA), and use the bovine serum albumin peptide LVNELTEFAK as the internal standard to detect the cashmere signature peptide YAVPVVTVSSPE and the fine wool signature peptide TCCEPTVCQSTCYQPTPCVSSPVR or SHSAWSILPR relative to the mass spectrum signal of the internal standard, according to the identification results to determine whether the tested variety contains cashmere or fine wool and the relevant content of cashmere.
[0073] 3. Results
[0074] like Figure 6~7 As shown, YAVPVVTVSSPE, the signature peptide of cashmere components in test products S1 and S2, was positive for identifi...
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