Method for improving efficiency of infecting host larvae with cordyceps sinensis
A technology for the host of Cordyceps sinensis and Cordyceps sinensis fungus is applied in the field of improving the efficiency of insect-borne fungi infecting insects, and can solve the problems of difficulty in repairing host larvae, difficulty in repairing host larvae, and environmental pollution in cultivation, so as to improve efficiency, reduce bacterial infection, Improve sloppy effect
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Embodiment 1
[0013] Take commercially available 5000U / mg lysozyme 0.2g and add it into 1000mL pure water, use disodium hydrogen phosphate and citric acid to adjust the pH value to 3.4, and prepare 1×10 3 U / mL of lysozyme solution. Spray the prepared lysozyme solution at a temperature of 10°C on the soil matrix where the Cordyceps sinensis host larvae grow, spray 0.1 mL of the lysozyme solution per cubic centimeter, and then put the Cordyceps sinensis host larvae into the pretreated soil matrix, Put 1 Cordyceps sinensis host larva per 9 square centimeters, raise it for 2 days under the environmental conditions of temperature of 10°C and humidity of 40%, and then let the liquid Cordyceps sinensis strain obtained from fermentation be applied to the body surface of the host larvae to make it contact with the Cordyceps sinensis strain At the same time, the host larvae without lysozyme pretreatment were contacted with Cordyceps sinensis strains in the same way as a control, and blood lymph fluid...
Embodiment 2
[0015] Take commercially available 10000U / mg lysozyme 5g and add it into 1000mL pure water, use disodium hydrogen phosphate and citric acid to adjust the pH value to 5.6, and prepare 5×10 4 U / mL of lysozyme solution. Spray the prepared lysozyme solution at 18°C on the soil matrix where the Cordyceps host larvae grow, spray 0.5 mL of the lysozyme solution per cubic centimeter, and then put the Cordyceps host larvae into the pretreated soil matrix, Put 1 Cordyceps sinensis host larva per 25 square centimeters, raise it for 7 days under the environmental conditions of 18°C and 90% humidity, then let the liquid Cordyceps sinensis strain obtained by fermentation be sprayed on the substrate where the host larvae grow, so that it can be mixed with Cordyceps sinensis fungus contact with the host larvae without lysozyme pretreatment at the same time with the Cordyceps fungus in the same way as a control, and 2 weeks after the contact, blood lymph fluid was taken for microscopic exa...
Embodiment 3
[0017] Take commercially available 10000U / mg lysozyme 0.5g and add it into 1000mL pure water, use disodium hydrogen phosphate and citric acid to adjust the pH value to 5.0, and prepare 5×10 3 U / mL of lysozyme solution. Spray the prepared lysozyme solution with a temperature of 12°C on the soil matrix where the Cordyceps sinensis host larvae grow, put 1 Cordyceps sinensis host larva per 18 square centimeters, spray 0.25 mL of the lysozyme solution per cubic centimeter, and then put Cordyceps sinensis The host larvae are put into the pretreated soil matrix and fed for 3 days at a temperature of 12°C and a humidity of 70%. Then the liquid Cordyceps strains obtained from fermentation are applied to the surface of the host larvae to make them contact with the Cordyceps strains At the same time, the host larvae without lysozyme pretreatment were contacted with Cordyceps sinensis strains in the same way as a control, and blood lymph fluid was taken 2 weeks after the contact to check ...
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