Preparation method for curcumin-loaded nano-emulsion

A technology for carrying curcumin and nanoemulsion is applied in the field of preparation of carrying curcumin nanoemulsion, which can solve the problems of hidden safety hazards and high cost, and achieve the effects of reducing preparation cost, low production cost and good emulsifying performance.

Active Publication Date: 2017-08-11
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention solves the technical problems of potential safety hazards and high cost in the existing discl...

Method used

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  • Preparation method for curcumin-loaded nano-emulsion
  • Preparation method for curcumin-loaded nano-emulsion
  • Preparation method for curcumin-loaded nano-emulsion

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specific Embodiment approach 1

[0018] The specific embodiment one, the preparation method of carrying curcumin nanoemulsion in the present embodiment is realized according to the following steps:

[0019] Step 1: 1.5 g of β-conglycinin was dissolved in 100 mL of deionized water, stirred for 6 hours, and ultrasonically treated at 200 W for 15 minutes to obtain an aqueous phase. The solubility of β-conglycinin in the water phase is improved by ultrasonic treatment, so that the emulsifying ability of the emulsifier (β-conglycinin) in the water phase is improved, which is beneficial to the formation of nanoemulsion.

[0020] Step 2: Mix 20mL of the water phase with 0.618mL of the oil phase, then place it in a high-speed shear homogenizer, homogenize at a speed of 10,000r / min for 2min, then place it in an ultrasonic cell pulverizer, and treat it with a power of 400W for 15min. Promptly obtain carrying curcumin nanoemulsion.

[0021] Wherein, the oil phase in step 2 is prepared according to the following steps: ...

specific Embodiment approach 2

[0022] Specific embodiment two, the preparation method of carrying curcumin nanoemulsion in the present embodiment is to realize according to the following steps:

[0023] Step 1. Dissolve 1.5g of β-conglycinin in 100mL of deionized water and stir for 6 hours, add alkaline protease, enzymatically hydrolyze at pH 9.0 and 45°C, freeze-dry, dissolve in water, and obtain a concentration of 15mg / mL of the aqueous phase. The amount of alkaline protease added is 5000U / g.

[0024] Step 2: Mix 20mL of the water phase with 0.618mL of the oil phase, then place it in a high-speed shear homogenizer, homogenize at a speed of 10,000r / min for 2min, then place it in an ultrasonic cell pulverizer, and treat it with a power of 400W for 15min. Promptly obtain carrying curcumin nanoemulsion.

[0025] Wherein, the oil phase in step 2 is prepared according to the following steps: dissolve 0.1 g of curcumin in 10 mL of medium-chain triglycerides, stir well, and centrifuge to remove insoluble matte...

specific Embodiment approach 3

[0026] Specific embodiment three, the preparation method of carrying curcumin nanoemulsion in the present embodiment is realized according to the following steps:

[0027] Step 1. Dissolve 1.5g β-conglycinin in 100mL PBS (0.02M, pH 9.0) buffer solution to obtain a β-conglycinin solution, then add dextran DX-40, fully dissolve, and store at 90°C After 80 minutes of reaction, freeze-dry and dissolve in water to obtain an aqueous phase with a concentration of 15 mg / mL. Dextran DX-40 and β-conglycinin solution were added to β-conglycinin solution at a mass ratio of 1:1.

[0028] Step 2: Mix 20mL of the water phase with 0.618mL of the oil phase, then place it in a high-speed shear homogenizer, homogenize at a speed of 10,000r / min for 2min, then place it in an ultrasonic cell pulverizer, and treat it with a power of 400W for 15min. Promptly obtain carrying curcumin nanoemulsion.

[0029] Wherein, the oil phase in step 2 is prepared according to the following steps: dissolve 0.1 g ...

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Abstract

The invention provides a preparation method for a curcumin-loaded nano-emulsion, belonging to the technical field of curcumin nano-emulsions. The preparation method provided by the invention overcomes the technical problems of potential safety hazards and high cost of conventional preparation methods for curcumin-loaded nano-emulsions. The preparation method comprises the following steps: 1, subjecting beta-conglycinin to ultrasonic treatment, enzymatic hydrolysis or glycosylation so as to prepare a water phase; and 2, mixing the water phase with an oil phase which is prepared from curcumin and middle-chain triglyceride, carrying out homogenization, and then carrying out treatment in an ultrasonic cell disruptor so as to obtain the curcumin-loaded nano-emulsion. The curcumin-loaded nano-emulsion prepared by using the method in the invention has a high entrapment rate. The method provided by the invention reduces preparation cost and can guarantee that the prepared curcumin-loaded nano-emulsion is healthy, safe and excellent in quality; and the method is more suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of curcumin nanoemulsion, and specifically relates to a preparation method for carrying curcumin nanoemulsion. Background technique [0002] Curcumin is an acidic polyphenolic compound extracted from the rhizomes of turmeric plants such as turmeric, zedoary, calamus, turmeric, etc. and other pharmacological activities. [0003] However, curcumin is a lipophilic substance with poor water solubility, and its bioavailability is low when it is taken into the body in the form of solid particles, suspensions, etc., and it is difficult to achieve the desired health care effect. [0004] There are still deficiencies in the existing disclosed methods for preparing nanoemulsions carrying curcumin. For example, the preparation of nanoemulsions by low-energy emulsification requires the use of a large amount of emulsifiers. After the nanoemulsion of curcumin is put into production and commercialized, there are potentia...

Claims

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Application Information

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IPC IPC(8): A61K9/107A61K31/12A61K47/42A61P29/00A61P31/04A61P35/00A61P39/06A61P1/16A61P3/06A61P31/10
CPCA61K9/1075A61K31/12A61K47/42
Inventor 许晶赵青山张晓松王笑宇陈子净
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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