Method of separating purification saponin glycosides composition from achyranthes longifolia makino
A technology of separation and purification, saponins, applied in the field of natural medicinal chemistry, can solve the problems of few research reports on the components of Achyranthes chinensis
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Embodiment 1
[0040] In order to further clarify the chemical components and active ingredients in the Yi medicine Achyranthes bidentata, obtain reference substances, establish the quality standards of the medicinal material Achyranthes bidentata and its prescription preparations, and clarify the material basis of the pharmacological effects of Achyranthes bidentata, this example studies the medicinal materials of Achyranthes bidentata. The chemical composition has been studied systematically.
[0041] 1 Instruments and materials
[0042] 1.1 Instrument
[0043] Waters Acquity UPLC H-Class System ultra-high performance liquid chromatography (Waters, USA); METTLER AE240 electronic analytical balance (Mettler-Toledo (Shanghai) Instrument Co., Ltd.); KQ-250B ultrasonic cleaner (Kunshan City) Ultrasonic Instrument Co., Ltd.); IKA RV10 rotary evaporator (German IKA company); SHB-Ⅲ circulating water vacuum pump (Gongyi Yingyu Huake Instrument Factory); digital constant temperature water bath HH-...
Embodiment 2
[0072] Instruments and materials
[0073] 1.1 Instrument
[0074]Waters Acquity UPLC H-CLass System ultra-high performance liquid chromatography (Waters, USA); METTLER AE240 electronic analytical balance (Mettler-Toledo (Shanghai) Instrument Co., Ltd.); IKARV10 rotary evaporator (IKA, Germany); KQ-250B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); SHB-Ⅲ circulating water vacuum pump (Gongyi Yingyu Huake Instrument Factory); digital constant temperature water bath HH-2 (Guohua Electric Co., Ltd.).
[0075] 1.2 Materials and reagents
[0076] The source of medicinal materials is shown in Table 4. It was identified by Professor Liu Yuan of Southwest University for Nationalities as the dry root of CyatHulaCapitata (Wall.) Moq., a plant of the family Amaranthaceae. -O-[α-L-rhamnopyranose (1→3)-β-D-glucopyranose]-oleanolic acid (HNX-3 for short) and 3-O-[α-L- Rhamnopyranose (1→3)-β-D-glucopyranose]-oleanolic acid-28-O-(β-D-glucopyranose) (referred to as HNX-5), fo...
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