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Method for rapidly breeding of tallow tree plants

A high-speed Chinese tallow tree technology, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of complex and cumbersome operation process, difficult to meet the demand for high-quality Chinese tallow tree seedlings, high production cost, etc., and achieve simple operation method and plant saving High quality material, low cost effect

Inactive Publication Date: 2017-08-15
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the method of tissue culture avoids the influence and limitation of natural factors such as breeding seasons and regions, the entire in vitro regeneration process of plants requires a strict aseptic environment, and the operation process is complicated and cumbersome, and the production cost is high
Therefore, it is also difficult to meet the needs of large-scale afforestation projects for high-quality Chinese tallow tree seedlings by tissue culture techniques.

Method used

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  • Method for rapidly breeding of tallow tree plants
  • Method for rapidly breeding of tallow tree plants
  • Method for rapidly breeding of tallow tree plants

Examples

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Effect test

Embodiment 1

[0034] A method for rapidly breeding black tallow plants of the present embodiment, comprising the following steps:

[0035] (1) Take material

[0036] In June, the micro-stem segments of the current-year branches of the tallow fine line (the tallow tree line with one or more characteristics of high yield, high quality and good stress resistance) were selected as explants;

[0037] (2) Pretreatment of explants

[0038] The micro stem section in the step (1) is cut into 1.5cm long, at least with 1 axillary bud and 1 blade of black tallow micro stem section, then placed under running water and rinsed, rinsed through potassium permanganate solution after 5min The potassium permanganate disinfection solution with a concentration of 0.1% was used for surface disinfection for 2 minutes, and during disinfection, the surface solution of the micro-stem segment was sucked dry and used for later use;

[0039] (3) Induction treatment of adventitious roots of explants

[0040] The morph...

Embodiment 2

[0046] A method for rapidly breeding black tallow plants of the present embodiment, comprising the following steps:

[0047] (1) Take material

[0048] In June, the micro-stem segments of the current-year branches of the tallow fine line (the tallow tree line with one or more characteristics of high yield, high quality and good stress resistance) were selected as explants;

[0049] (2) Pretreatment of explants

[0050] The micro stem section in the step (1) is cut into 2.0cm long, at least with 1 axillary bud and 1 blade of black tallow micro stem section, then placed under running water and rinsed, rinsed by potassium permanganate solution after 10min Potassium permanganate disinfection solution with a concentration of 0.15% was used for surface disinfection for 5 minutes, and during disinfection, it was continuously stirred, and the surface solution of the micro-stem segment was sucked dry after disinfection, and it was used for later use;

[0051] (3) Induction treatment ...

Embodiment 3

[0058] A method for rapidly breeding black tallow plants of the present embodiment, comprising the following steps:

[0059] (1) Take material

[0060] In June, the micro-stem segments of the current-year branches of the tallow fine line (the tallow tree line with one or more characteristics of high yield, high quality and good stress resistance) were selected as explants;

[0061] (2) Pretreatment of explants

[0062] The micro stem section in the step (1) is cut into 3.0cm long, at least with 1 axillary bud and 1 blade of black tallow micro stem section, then placed under running water and rinsed, rinsed by potassium permanganate solution after 15min Potassium permanganate disinfection solution with a concentration of 0.2% was used for surface disinfection for 6 minutes, and during disinfection, the surface solution of the micro-stem segment was sucked dry and used for later use;

[0063] (3) Induction treatment of adventitious roots of explants

[0064] The morphological...

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Abstract

The invention discloses a method for rapid breeding of Chinese tallow tree plants, which comprises the following steps: taking materials, pretreatment of explants, induction treatment of adventitious roots of the explants, colonization of the explants, and management of the seedling formation of the explants. The present invention has the advantages of: firstly, germplasm materials are saved, and reproductive efficiency is improved; secondly, materials are conveniently obtained, and materials are abundant, and the produced plants can maintain the original excellent traits of female plants; thirdly, the speed of obtaining seedlings is fast , rapid propagation speed; fourth, avoiding the influence and limitation of natural factors such as seasons and regions, and also avoiding the tediousness of the process of tissue culture and breeding of Chinese tallow tree and the defect of low survival rate of subsequent tissue culture regenerated seedlings domestication and transplanting; fifth, The cost of producing regenerated seedlings is low, and the operation method is simple, which is suitable for the industrialization of Chinese tallow tree seedlings; Sixth, strictly optimize and control the composition and concentration of the rooting promotion liquid; Seventh, the type and concentration selection of disinfectants, nutritional supplements, and nutritional substrates It also strictly cooperates with the growth process of Chinese tallow tree.

Description

technical field [0001] The invention relates to the field of tallow propagation, in particular to a method for rapidly breeding tallow plants. Background technique [0002] Tallow (Sapindus mukorossi Gaertn.) is a deciduous tree of the Euphorbiaceae family. It is a multifunctional tree species that integrates ornamental, greening, medicinal, soil and water conservation and biodiesel production. The tallow tree is beautiful in shape, and its leaves show red, orange, yellow, green, purple and other colors after autumn. It is a landscape tree species that integrates fruit, leaves, and shape. An ideal raw material for biodiesel. In addition, tallow is an ideal tree species for afforestation on barren hills because of its well-developed root system, rapid growth, and low soil requirements. It is also of great value in greening and soil and water conservation. In recent years, with the improvement of the comprehensive development and utilization value of black tallow, the demand...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴丽芳侯金艳
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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