Sea backthern leaf tissue culture method
A technology of tissue culture and leaves, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of rapid propagation of seabuckthorn leaves that have not yet appeared, and achieve the effect of high seedling survival rate
Inactive Publication Date: 2017-08-15
新疆阿勒泰地区园林场
View PDF2 Cites 8 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, there is no method for rapid propagation using seabuckthorn leaves as materials
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0016] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.
[0017] A seabuckthorn leaf tissue culture method, comprising the following steps:
[0018] Step 1, primary culture: take 40-day-old test-tube seedling leaves and cut them into 2.5-3.0cm 2 Inoculate the small pieces of the callus on the callus induction medium, 10-15 leaves per bottle; the culture temperature is about 25°C, the humidity is 50%-70%, the light intensity is 2000-3000Lx, and the light time is 13-16h / d; Observe and record once every 3-5 days;
[0019] The formula of the callus induction medium is 1 / 2B5+6-BA0.5mg / L+IAA0.3mg / L+agar 0.6%+sucrose 3%;
[0020] Step 2, subculture propagation: get the rootless seedlings cultivated in the first generation and cut them into stem tips and stem segments with axillary buds and inoculate them on the proliferation medium respectively;
[0021] The formula of the proliferation medium is 1 / 2B5+6-BA0....
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a sea backthern leaf tissue culture method, which comprises the following steps of 1, primary generation culture: taking 40-day-old test tube seedling leaves; cutting the leaves into small pieces being 2.5 to 3.0 cm<2>; inoculating the small pieces onto a callus induction culture medium; 2, subculture multiplication: taking rootless seedlings subjected to the primary generation culture; cutting the rootless seedlings subjected to primary generation culture into stem tips; respectively inoculating the steam sections with axillary buds onto an enrichment culture medium; 3, rooting culture: taking the rootless seedlings subjected to subculture multiplication; inoculating the rootless seedlings subjected to subculture multiplication onto the rooting culture medium; 4, transplanting: when the height of the rooting test tube seedlings is 5.0cm, moving out of the whole bottle of the rooting test tube seedlings out of the culture room; putting the seedlings in a room temperature shady place for 4 to 5 days; opening a bottle cover; performing placement for 4 to 5 days; taking out the test tube seedlings; slightly washing root part agar by tap water; transplanting the seedlings into a substrate subjected to high-temperature sterilization in advance. The sea backthern leaf tissue culture method has the advantages that the sea backthern aseptic seedling leaves are used as materials; the proper culture medium formula in each culture stage and the proper transplanting substrate are selected; high young seedling survival rate is realized; the basis is provided for the factorized production.
Description
technical field [0001] The invention relates to the technical field of tissue culture of fruit crops, in particular to a method for tissue culture of seabuckthorn leaves. Background technique [0002] Seabuckthorn (Hippophae rhamnoides L.) is a plant of the genus Hippophae L. in the family Elaeagnaceae. It is a tree species with strong adaptability and has a wide range of ecological and social benefits. There are many breeding methods, and conventional forest tree breeding methods can be used, but only specific breeding methods can be selected under the premise of large-scale production and quality assurance. Preliminary studies on the rapid propagation of seabuckthorn have been carried out by using stem segments, internodes, and shoot tips with axillary buds, and many successful reports have been made. But there is no method for rapid propagation using seabuckthorn leaves as materials. Contents of the invention [0003] The object of the present invention is to provide ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 赵英徐航郑兴国韩晓燕刘伟张志刚
Owner 新疆阿勒泰地区园林场