A method and device for controlling cell concentration uniformity

Abstract

The invention discloses a method and device for mixing cell culture fluid and cell mixed liquid and controlling cell concentration uniformity in mixed liquid. The bottom of a cell fluid mixing tank is fixedly connected with the upside of the center of a horizontally arranged base plate of a vibration table, a circulation hose is connected between the bottom and the top of a cell fluid mixing tank, the circulation hose is provided with a peristaltic pump, the circulation hose between the peristaltic pump and the top of the cell fluid mixing tank is connected with the top of an image acquisition device through a sampling hose, a circular hollow hole penetrated up and down is arranged on the surface of a turntable, close to an edge, in a shell of the image acquisition device, when the turntable is in an initial position, the circular hollow hole is directly below an outlet of the sampling hose, when the turntable rotates a stepping angle counterclockwise and stops, a first sample droplet of the sampling hose is dropped on the upper surface of the turntable, and an image sensor is arranged directly below the first sampling droplet. According to the method and device for mixing the cell culture fluid and the cell mixed liquid and controlling the cell concentration uniformity in the mixed liquid, cells are mixed with a culture solution more fully and the uniformity of the cell mixed liquid can be judged.

Description

technical field [0001] The invention relates to the field of liquid mixing, specifically mixing cell culture liquid and cell mixed liquid, controlling the uniformity of cell concentration in the mixed liquid, and the mixed liquid whose uniformity meets the requirements can further flow into the microfluidic chip for further processing. detection. Background technique [0002] The microfluidic chip integrates the functions of traditional laboratories into a small chip, and realizes basic operations such as reaction, separation, and inspection involved in the fields of biology and chemistry. The pipe size of the microfluidic chip is on the order of μm, which is comparable to the size of the cell. The sample volume of the microfluidic chip only needs a very small few microliters. Therefore, the cell mixture flowing into the microfluidic chip must be mixed efficiently to make the microfluidic chip The cells in the fluidic chip are evenly distributed for experimentation and obse...

AI Technical Summary

Problems solved by technology

How to achieve efficient mixing of cell culture fluid at the microscopic scale is a difficult problem in the field of microfluidic cell detection. At present, there are few mixers for cell solutions, and most of them are manually mixed or use liquid mixers.

However, the liquid mixer is not suitable for mixing the cell solution, because the cell membrane is easy to break, the stirring equipment cannot be used and the external force cannot be too large, and secondly, too much air cannot be mixed in, which will pollute the cell mixture, and whether the solution is fully mixed, the existing The Mixer is not yet detectable

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