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Apparatus and methods for carrying out chemical reactions

A chemical reaction and reaction technology, applied in biochemical equipment and methods, chemical instruments and methods, specific-purpose bioreactors/fermenters, etc., can solve the problems of limited PCR amplification speed and other issues

Active Publication Date: 2021-07-30
COYOTE BIOSCIENCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, in many cases, the speed of PCR amplification is limited by the performance of the instrument (e.g., thermal cycler) rather than the biological reaction itself

Method used

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  • Apparatus and methods for carrying out chemical reactions
  • Apparatus and methods for carrying out chemical reactions
  • Apparatus and methods for carrying out chemical reactions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0271] Example 1 - Rapid Thermal Cycling

[0272] The user loads 20 sample volumes including the DNA sample, PCR reagents, and intercalating dye into the sample holder of the thermal cycler. Each sample volume is 20 mL. The timing control system of the thermal cycler controls the movement of the sample holder and four thermal zones mounted on a rotating arm. The thermal cycler begins thermal cycling the sample volume. During each thermal cycle, the following happens:

[0273] A) The sample holder is moved downwards while the first overshoot hot zone maintained at a constant temperature of 135°C is moved in horizontally so that the sample holder and the first overshoot hot zone come into thermal contact with each other. The sample volume in the sample holder is heated to about 95°C. The sample holder moves upward while the first overshoot hot zone moves out horizontally.

[0274] B) The sample holder is moved down a second time while the first target thermal zone maintaine...

Embodiment 2

[0278] Example 2 - constant temperature area of ​​metal bath

[0279] A 200 microliter (μL) PCR sample tube was loaded with 50 μL of a liquid solution containing the sample and PCR reagents at room temperature. Configure four metal bath hot zones as follows: the first overshoot hot zone kept constant at 135°C, the first target hot zone kept constant at 95°C, the second overshoot hot zone kept constant at 8°C and A second target thermal zone of 55°C. The PCR tubes are moved in a cycle between the first overshoot thermal zone, the first target thermal zone, the second overshoot thermal zone, and the second target thermal zone. The PCR tube and its contents were heated and cooled between target temperature levels of 95°C and 55°C at a rate of about 7°C / sec.

Embodiment 3

[0280] Example 3 - Operation from Motor Vehicle Power Supply

[0281] The user brings the thermal cycler device into the vehicle. The user plugs the thermal cycler device's power supply into the vehicle's cigarette lighter power adapter. The user loads the sample and reagents into the thermal cycler device, which mixes to form a reaction mixture. The user sets the thermal cycler device to run. The thermal cycler device draws power from a motor vehicle and cycles the temperature of the reaction mixture. The sample is amplified and the results of the amplification are recorded.

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Abstract

Provided herein are devices, systems and methods for performing nucleic acid amplification. The devices, systems and methods are suitable for portability, fast operation, low power consumption, integrated operation and remote monitoring.

Description

[0001] cross reference [0002] This application claims priority to PCT Application Serial No. PCT / CN2014 / 095987 filed on December 31, 2014 and PCT Application Serial No. PCT / CN2015 / 074513 filed on March 18, 2015, which are incorporated by reference in their entirety here. Background technique [0003] Polymerase chain reaction (PCR) is a widely used technique in molecular biology for the amplification of nucleic acid molecules. PCR relies on thermal cycling, cycles of repeated heating and cooling of a reaction mixture. These cycles allow for the melting and enzymatic replication of nucleic acids. [0004] In an appropriate reagent reaction system, polymerases can rapidly perform chain reactions. Indeed, each cycle of amplification of nucleic acid molecules in the polymerase chain reaction (PCR) can occur within one to two seconds, or even less than one second. Thus, in many cases, the speed of PCR amplification is limited by the performance of the instrument (eg, thermal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/38C12Q1/70C12Q1/686F27D11/00
CPCB01L7/52B01L9/523B01L2200/026B01L2300/023B01L2300/024B01L2300/027B01L2300/0829B01L7/5255B01L2200/025B01L2300/18B01L2300/1894C12Q1/686
Inventor 李响慕晓兵李晨冯慧颖周阳韩玉光陈娟胡亚超
Owner COYOTE BIOSCIENCE CO LTD
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