Application and preparation of β-sitosterol
A sitosterol and plant technology, applied in the application of beta-sitosterol and its preparation field, can solve problems such as unresearched
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Embodiment 1
[0017] Example 1 Screening of β-sitosterol root application concentration
[0018] Test material: The widely used ‘Latino’ white clover was used as the test material
[0019] Material cultivation: first soak the white clover seeds with 0.1% potassium permanganate solution for 10 minutes to sterilize, and rinse with deionized water 3 times, press 1g / m 2 Sow the seeds in a plastic pot with a length of 20cm, a width of 15cm, and a height of 5cm filled with quartz sand, and place them in a light incubator for germination (the temperature is 23 / 19°C during the day / night; the duration is 12h each; relative Humidity is 70%; light intensity is 700μmol m -2 s -1 ). After the seeds germinated 7 days later, the seedlings were cultured with Hoagland nutrient solution to 30 days, and materials with consistent growth were selected for subsequent experiments.
[0020] Experimental design: A total of 7 β-sitosterol concentration gradients were set up, respectively: 0mg / L (control, direct drought st...
Embodiment 2
[0025] Example 2 Screening of concentration of β-sitosterol sprayed on foliage
[0026] Test material: The drought-sensitive'Latino' white clover was used as the test material.
[0027] Material cultivation: Mix the garden soil and nutrient soil at a ratio of 1:1, add insecticides and fungicides, and mix them evenly. Place them outdoors for 3 days before use. Plant white clover in a pot with a diameter of 20cm and a depth of 25cm. Fill the pot with the same amount of mixed soil at a rate of 1g / m 2 Sow the white clover seeds at the planting rate, cover with shallow soil and water. The materials are placed in the greenhouse for 3 months, and 250mL of Hoagland's total nutrient solution is poured in each pot every week, and routine management such as fertilization, weeding, watering, and pests and diseases is uniformly performed. The average temperature of the greenhouse is 23℃, the relative humidity is 65%, the light time is 12h, and the light intensity is 500μmol m -2 s -1 . After ...
Embodiment 3
[0033] Example 3 Effect of Foliar Spraying β-Sitosterol on Drought Resistance Physiological Index of White Clover
[0034] Test material: Drought-sensitive'Latino' white clover was used as the test material
[0035] Material cultivation: same as Example 2
[0036] Experimental design: According to experimental example 2, the best leaf spraying concentration of 15mg / L is the spraying concentration of this experiment. The experiment has 4 treatments, and each treatment is set up 4 independent repeats, respectively: ① Normal condition control (CK ): Foliar sprayed with solvent without β-sitosterol and cultured in the greenhouse for 18 days as a control; ②CK+β-sitosterol: foliar sprayed with 15mg / L β-sitosterol and cultured in the greenhouse 18 days; ③Direct drought stress: After spraying the solvent without β-sitosterol on the leaf surface, the natural drought in the greenhouse for 18 days; ④Drought + β-sitosterol: After spraying 15mg / L β-sitosterol on the leaf surface Natural drought...
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