Detection and application of prawn enterocytozoon hepatopenaei trehalose-6-phosphate synthase gene

Abstract

The invention provides detection and application of a prawn enterocytozoon hepatopenaei trehalose-6-phosphate synthase gene and relates to prawn enterocytozoon hepatopenaei. Reverse transcription is performed through extracting the total RNA of a prawn infected with the prawn enterocytozoon hepatopenaei, and then the one trehalose-6-phosphate synthase gene is identified through specific primer amplification. According to gene sequence information, an optimal primer is designed and used for a real-time quantitative PCR method, so that a basis is provided for detecting a transcription expression level of the gene in a prawn. The transcription level of the prawn enterocytozoon hepatopenaei trehalose-6-phosphate synthase gene in the prawn is detected through the real-time quantitative PCR method, and the real-time quantitative PCR method can be used in the detection of prawn enterocytozoon hepatopenaei diseases. The method has the characteristics of being high in sensitivity and good in specificity, and the information and the method are provided for prawn disease prevention and control.

Description

technical field [0001] The invention relates to prawn hepatic enterozoa, in particular to the detection and application of the prawn hepatic enterozoa trehalose-6-phosphate synthase gene. Background technique [0002] Enterocytozoon hepatopenaei (EHP), also known as Enterocytozoa, is a microsporidia found in prawns in recent years, mainly infecting important cultured shrimps such as Litopenaeus vannamei and Penaeus monodon [1] . It was first isolated and named in 2009 from the slow-growing tiger prawn (Penaeus monodon ) farmed in Thailand [2] . [0003] In recent years, a high number of EHP-positive shrimps have been detected in shrimp seedlings and cultured shrimps in Zhejiang, Guangdong, Fujian and other provinces in my country, and the detection rate is increasing year by year. [3] . Shrimp infection with E. hepatica generally does not cause immediate death. Shrimp can eat normally, with obvious abnormalities in body color, intestines, and stomach. Some infected shrim...

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Problems solved by technology

However, these detection methods also have shortcomings, including only qualitative description of the pathogenic microorganism, incapable of quantitative research, insufficient sensitivity and specificity, etc. [6]

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