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TLC (Thin layer chromatography) rapid identification method for cacumen biotae

An oriental orientalis leaf and fast technology is applied in the field of TLC rapid identification of oriental orientalis leaves, which can solve the problems such as the inability to meet the needs of quickness and simplicity, the large amount of organic reagents, the cumbersome operation steps, etc., and achieves the reduction of discomfort and danger. Reproducible, chromatographically clear results

Inactive Publication Date: 2017-09-05
SHANDONG ACAD OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the current 2015 edition of the Chinese Pharmacopoeia contains the legal TLC identification method for Arborvitae leaves, but due to the Soxhlet extraction step used in this method, it takes at least 6 hours, and the heating and reflux step also takes 1 hour. The actual operation In the process, there are disadvantages such as cumbersome operation steps, long operation time, and high cost due to the large amount of organic reagents, which cannot meet the needs of fast and simple in practical applications.

Method used

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  • TLC (Thin layer chromatography) rapid identification method for cacumen biotae
  • TLC (Thin layer chromatography) rapid identification method for cacumen biotae
  • TLC (Thin layer chromatography) rapid identification method for cacumen biotae

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Effect test

Embodiment 1

[0028] A kind of fast TLC identification method of orientalis leaves, the steps comprising:

[0029] (1) Take 3g of the sample powder to be tested, add 25mL of water, add 3mL of hydrochloric acid, add 40mL of ethyl acetate, heat and reflux in a water bath for 30min, and filter while it is hot. 3 times, 10mL each time, evaporate to dryness in a water bath, add 5mL of methanol to dissolve the residue, and use it as the test solution;

[0030] (2) Separately take quercetin standard substance and add ethanol to prepare a 0.1mg / mL solution as the reference solution;

[0031] (3) According to the 2015 edition of the Chinese Pharmacopoeia Part IV 0502 thin-layer chromatography test, draw 3 μL of the test solution and the reference solution, respectively, and spot on the same silica gel G thin-layer plate, with toluene at a volume ratio of 5:2:1 -The upper layer solution of ethyl acetate-formic acid was developed with a developing agent, taken out, dried in the air, sprayed with 1wt%...

Embodiment 2

[0034] A kind of fast TLC identification method of orientalis leaves, the steps comprising:

[0035] (1) Take 2g of the sample powder to be tested, add 20mL of water, add 2mL of hydrochloric acid, add 30mL of ethyl acetate, heat and reflux in a water bath for 20min, filter while hot, put the filtrate in a separatory funnel, separate the liquid, take the ethyl acetate layer, wash with water 3 times, 10mL each time, evaporate to dryness in a water bath, add methanol 4mL to dissolve the residue, and use it as the test solution;

[0036] (2) Separately take quercetin standard substance and add ethanol to prepare a 0.05 mg / mL solution as the reference solution;

[0037] (3) According to the 2015 edition of the Chinese Pharmacopoeia Part IV 0502 thin-layer chromatography test, draw 2 μL each of the test solution and the reference solution, and spot them on the same silica gel G thin-layer plate, and use toluene with a volume ratio of 4:2:1 -The upper layer solution of ethyl acetate...

Embodiment 3

[0040] A kind of fast TLC identification method of orientalis leaves, the steps comprising:

[0041] (1) Take 4g of the sample powder to be tested, add 30mL of water, add 4mL of hydrochloric acid, add 50mL of ethyl acetate, heat and reflux in a water bath for 40min, filter while hot, put the filtrate in a separatory funnel, separate the liquid, take the ethyl acetate layer, wash with water 3 times, 10mL each time, evaporate to dryness in a water bath, add 6mL of methanol to dissolve the residue, and use it as the test solution;

[0042] (2) Separately take quercetin standard substance and add ethanol to prepare a 0.15 mg / mL solution as the reference solution;

[0043](3) According to the 2015 edition of the Chinese Pharmacopoeia Part IV 0502 thin-layer chromatography test, draw 3 μL of the test solution and the reference solution, respectively, on the same silica gel G thin-layer plate, and use toluene with a volume ratio of 6:2:1 -The upper layer solution of ethyl acetate-fo...

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Abstract

The invention relates to a TLC (Thin layer chromatography) rapid identification method for cacumen biotae. The method comprises the following steps: taking a to-be-detected sample, adding water, hydrochloric acid and ethyl acetate, heating, refluxing and hydrolyzing in a water bath, filtering while hot, separating the filtrate, washing, evaporating to dryness, adding methanol into the residues for dissolving, and taking the obtained solution as a test solution; and adopting thin layer chromatography, and viewing under an ultraviolet lamp. The method has the advantages of high efficiency, convenience, good reproducibility, clear chromatography, good separation of spots, and the like.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a TLC rapid identification method of oriental cypress leaves. Background technique [0002] Arborvitae leaves, also known as cypress leaves, cluster cypress leaves, cypresses, cloud cypresses, etc., are a good medicine for treating blood that doctors value. Traditional Chinese medicine believes that cypress leaves are bitter in taste, slightly cold in nature, non-toxic, and enter the heart, liver, and large intestine meridians. It has the functions of cooling blood, stopping bleeding, dispelling internal dampness, and dispelling swelling and poison. It can cure hematemesis, epistaxis, hematuria, bloody dysentery, metrorrhagia, rheumatic pain, cough, burns and other diseases. According to scientific research, Arborvitae leaves contain a large amount of volatile oil. The main components in the oil are arborvitene, fennelone, etc., and the leaves also contain var...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 石典花孙立立张军戴衍朋
Owner SHANDONG ACAD OF CHINESE MEDICINE
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