Nisin-grafted chitosan quaternary ammonium salt, method for preparing same and application of nisin-grafted chitosan quaternary ammonium salt

A technology of nisin and chitosan quaternary ammonium salt, which is applied in the field of polymer chemical materials, can solve problems such as side reactions and toxicity, and achieve the effects of low cost, environmental friendliness, good antioxidant capacity and antibacterial activity

Inactive Publication Date: 2017-09-22
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such chemical reagents inevitably produce toxicity and are often accompanied by some side reactions

Method used

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  • Nisin-grafted chitosan quaternary ammonium salt, method for preparing same and application of nisin-grafted chitosan quaternary ammonium salt
  • Nisin-grafted chitosan quaternary ammonium salt, method for preparing same and application of nisin-grafted chitosan quaternary ammonium salt
  • Nisin-grafted chitosan quaternary ammonium salt, method for preparing same and application of nisin-grafted chitosan quaternary ammonium salt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Prepare nisin grafted chitosan quaternary ammonium salt, the method is as follows:

[0030] Disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution with a concentration of 0.2mol / L were prepared respectively, and then the disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution were mixed uniformly at a volume ratio of 96:4 to obtain a PBS buffer with a pH of 4.0 Solution, measure 50mL of prepared PBS buffer solution with a pH of 4.0 into a three-necked flask, add 1.0g of chitosan quaternary ammonium salt to fully dissolve, then weigh 0.5g of nisin and 0.05g of transglutamin The enzyme freeze-dried powder was dissolved in 50 mL of PBS buffer solution with a pH of 4 to obtain a nisin solution and a transglutaminase solution, and the nisin solution and the transglutaminase solution were added to the three-necked flask successively, After stirring the reaction at 25° C. for 1 h, the reaction liquid was stirred in a boiling water b...

Embodiment 2

[0033] Prepare nisin grafted chitosan quaternary ammonium salt, the method is as follows:

[0034] Separately prepare disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution with a concentration of 0.2mol / L, and then mix the disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution at a volume ratio of 95:5 to prepare PBS buffer with a pH of 5.0 Solution, measure 50mL of prepared PBS buffer solution with a pH of 5.0 into a three-necked flask, add 1.0g of chitosan quaternary ammonium salt to fully dissolve, then weigh 1.0g of nisin and 0.1g of transglutamin Freeze-dried enzyme powder, dissolved in 50mL PBS buffer solution with pH 5.0 respectively to obtain nisin solution and transglutaminase solution, add nisin solution and transglutaminase solution to the three-necked flask in turn , after stirring and reacting at 35° C. for 2 h, the reaction liquid was placed in a boiling water bath and stirred for 15 min to inactivate the enzyme, and...

Embodiment 3

[0036] Prepare nisin grafted chitosan quaternary ammonium salt, the method is as follows:

[0037]Separately prepare disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution with a concentration of 0.2mol / L, and then mix the disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution at a volume ratio of 88:12 to prepare PBS buffer with a pH of 5.0 Solution, measure 50mL of prepared PBS buffer solution with a pH of 5.0 into a three-necked flask, add 1.0g of chitosan quaternary ammonium salt to fully dissolve, then weigh 1.5g of nisin and 0.15g of transglutamin Freeze-dried enzyme powder was dissolved in 50mL PBS buffer solution respectively to obtain nisin solution and transglutaminase solution. Add nisin solution and transglutaminase solution to the three-necked flask in turn, and set the temperature at 45°C. After reacting under stirring for 3 hours, the reaction solution was placed in a boiling water bath and stirred for 15 minutes to ...

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Abstract

The invention belongs to the field of high-polymer chemical materials, and particularly relates to nisin-grafted chitosan quaternary ammonium salt, a method for preparing the same and application of the nisin-grafted chitosan quaternary ammonium salt. Chitosan quaternary ammonium salt and nisin are used as substrate, transglutaminase is used as a catalyst, and the nisin-grafted chitosan quaternary ammonium salt is prepared by means of catalytic grafting. The nisin-grafted chitosan quaternary ammonium salt, the method and the application have the advantages that processes for preparing the nisin-grafted chitosan quaternary ammonium salt are simple and environmentally friendly and are low in cost; the nisin-grafted chitosan quaternary ammonium salt prepared by the aid of the method is good in water solubility and excellent in antioxidant capacity and antibacterial activity, is free of obvious toxicity for cells and has potential application value in the aspect of wound healing.

Description

technical field [0001] The invention belongs to the field of macromolecular chemical materials, and in particular relates to a nisin-grafted chitosan quaternary ammonium salt and a preparation method and application thereof. Background technique [0002] Chitosan (chitosan, CS) is the product of partial deacetylation of chitin under alkaline conditions. It is the only natural polysaccharide with obvious alkaline in nature. It has excellent biodegradability, good antibacterial performance and biophase Capacitive, but its insoluble properties under alkaline and neutral conditions limit its application to a large extent. The quaternization modification of chitosan can effectively improve the solubility of chitosan in neutral or even weakly alkaline aqueous solution. Compared with chitosan, the modified chitosan quaternary ammonium salt has more It has attracted widespread attention for its excellent water solubility, moisture absorption and antibacterial properties. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61K38/16A61P17/02A61P31/04
CPCC08B37/003A61K38/164
Inventor 樊李红闵莲柳梦朱晨
Owner WUHAN UNIV OF TECH
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