Nisin-grafted chitosan quaternary ammonium salt, method for preparing same and application of nisin-grafted chitosan quaternary ammonium salt
A technology of nisin and chitosan quaternary ammonium salt, which is applied in the field of polymer chemical materials, can solve problems such as side reactions and toxicity, and achieve the effects of low cost, environmental friendliness, good antioxidant capacity and antibacterial activity
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Embodiment 1
[0029] Prepare nisin grafted chitosan quaternary ammonium salt, the method is as follows:
[0030] Disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution with a concentration of 0.2mol / L were prepared respectively, and then the disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution were mixed uniformly at a volume ratio of 96:4 to obtain a PBS buffer with a pH of 4.0 Solution, measure 50mL of prepared PBS buffer solution with a pH of 4.0 into a three-necked flask, add 1.0g of chitosan quaternary ammonium salt to fully dissolve, then weigh 0.5g of nisin and 0.05g of transglutamin The enzyme freeze-dried powder was dissolved in 50 mL of PBS buffer solution with a pH of 4 to obtain a nisin solution and a transglutaminase solution, and the nisin solution and the transglutaminase solution were added to the three-necked flask successively, After stirring the reaction at 25° C. for 1 h, the reaction liquid was stirred in a boiling water b...
Embodiment 2
[0033] Prepare nisin grafted chitosan quaternary ammonium salt, the method is as follows:
[0034] Separately prepare disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution with a concentration of 0.2mol / L, and then mix the disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution at a volume ratio of 95:5 to prepare PBS buffer with a pH of 5.0 Solution, measure 50mL of prepared PBS buffer solution with a pH of 5.0 into a three-necked flask, add 1.0g of chitosan quaternary ammonium salt to fully dissolve, then weigh 1.0g of nisin and 0.1g of transglutamin Freeze-dried enzyme powder, dissolved in 50mL PBS buffer solution with pH 5.0 respectively to obtain nisin solution and transglutaminase solution, add nisin solution and transglutaminase solution to the three-necked flask in turn , after stirring and reacting at 35° C. for 2 h, the reaction liquid was placed in a boiling water bath and stirred for 15 min to inactivate the enzyme, and...
Embodiment 3
[0036] Prepare nisin grafted chitosan quaternary ammonium salt, the method is as follows:
[0037]Separately prepare disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution with a concentration of 0.2mol / L, and then mix the disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution at a volume ratio of 88:12 to prepare PBS buffer with a pH of 5.0 Solution, measure 50mL of prepared PBS buffer solution with a pH of 5.0 into a three-necked flask, add 1.0g of chitosan quaternary ammonium salt to fully dissolve, then weigh 1.5g of nisin and 0.15g of transglutamin Freeze-dried enzyme powder was dissolved in 50mL PBS buffer solution respectively to obtain nisin solution and transglutaminase solution. Add nisin solution and transglutaminase solution to the three-necked flask in turn, and set the temperature at 45°C. After reacting under stirring for 3 hours, the reaction solution was placed in a boiling water bath and stirred for 15 minutes to ...
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