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Microorganism bacterium agent of deodorant use for household garbage and cultivation feces and preparation method and application

A technology for microbial inoculants and domestic waste, applied in the field of environmental microorganisms, can solve the problems of not eliminating odorous substances from the source, secondary pollution of the environment, expensive raw materials, etc. amount of effect

Inactive Publication Date: 2017-09-22
BIOLAND ENVIRONMENTAL TECH GRP CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These products have achieved certain results in domestic waste and excrement deodorization, but generally have the following deficiencies: (1) first, these products rely on the addition of essences in some chemical reagents, which only play a certain masking effect on odorous substances. The generation of odorous substances (such as ammonia, hydrogen sulfide, etc.) is not eliminated from the source
(2) Secondly, chemical products, due to the characteristics of the raw materials themselves, have certain toxicity to the environment, and there is also a potential risk of secondary pollution to the environment
(3) Again, even if low-toxic plant extracts are used, there are also disadvantages of expensive raw materials and high production costs

Method used

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  • Microorganism bacterium agent of deodorant use for household garbage and cultivation feces and preparation method and application
  • Microorganism bacterium agent of deodorant use for household garbage and cultivation feces and preparation method and application
  • Microorganism bacterium agent of deodorant use for household garbage and cultivation feces and preparation method and application

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preparation example Construction

[0038] The invention provides a microbial agent for deodorizing household garbage and cultured feces, a preparation method and an application. The present invention selects and breeds Saccharomyces cerevisiae and S. acid Lactobacillus, and can effectively remove ammonia and hydrogen sulfide gas in domestic garbage and feces. Bacillus natto and Bacillus subtilis screened from feces materials can produce a large amount of neutral protease, protease can effectively decompose feces proteins to produce odorous organic nitrogen, and convert it into nitrate nitrogen, So as to play a fast deodorizing substance. The white rot fungi screened from municipal garden waste can effectively degrade lignin and other difficult-to-decompose substances, and can also degrade odorous gases such as organic sulfur.

[0039] The microbial bacterial agent of the invention has strong adaptability, strong deodorizing ability and high efficiency, and is suitable for deodorizing domestic garbage, farm ani...

Embodiment 1

[0055] Step 1: Preparation of initial strain

[0056] Collect samples from domestic waste landfills and farm animal manure respectively, dilute the samples 50 times with sterile water, shake them on a shaking table at 200 RPM (rev / min) for 1-2 hours, and then set the mass fraction as 5 % of the inoculum was respectively inserted into LB medium and PDA medium for 5 days, and this was repeated 5 times, and then the enrichment solution was diluted to 10 8 ~10 10 times, spread on LB and PDA selective plates, select a single colony with typical morphology, store it on the slant of the test tube, and send it for 16SRNA identification. Five strains with good deodorizing effects were screened out in our laboratory, A, B, C, D, and E. After identification, A was Saccharomyces cerevisiae, B was Lactobacillus acidophilus, C was Bacillus subtilis, and D was sodium Bacillus bean and E are white rot fungi.

[0057] Step 2: Activation

[0058] Activation of strains stored at 4°C on slant...

Embodiment 2

[0066] Step 1: Preparation of initial strain

[0067] Collect samples from domestic waste landfills and farm animal manure respectively, dilute the samples 50 times with sterile water, shake them on a shaking table at 200 RPM (rev / min) for 1-2 hours, and then set the mass fraction as 5 % of the inoculum was respectively inserted into LB medium and PDA medium for 5 days, and this was repeated 5 times, and then the enrichment solution was diluted to 10 8 ~10 10 times, spread on LB and PDA selective plates, select a single colony with typical morphology, store it on the slant of the test tube, and send it for 16SRNA identification. Five strains with good deodorizing effects were screened out in our laboratory, A, B, C, D, and E. After identification, A was Saccharomyces cerevisiae, B was Lactobacillus acidophilus, C was Bacillus subtilis, and D was sodium Bacillus bean and E are white rot fungi.

[0068] Step 2: Activation

[0069] Activation of strains stored at 4°C on slant...

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Abstract

The invention provides a microorganism bacterium agent of deodorant use for household garbage and cultivation feces and a preparation method and application. The microorganism bacterium agent is prepared from, by weight, 30-70 parts of saccharomyces cerevisiae, 10-60 parts of lactobacillus acidophilus, 5-30 parts of bacillus natto, 5-20 parts of bacillus subtilis, and 10-30 parts of white-rot fungi. The microorganism bacterium agent of deodorant use for household garbage and cultivation feces has the advantages that strain has the capability of inhibiting the growth and reproduction of rot bacteria, and provides the deodorization effect on odor substances from the source. The bacterium agent has a high number of efficient live bacteria, handles the same amount of materials, and is capable of reducing the dosage of bacterium agent and reducing greatly the operating costs.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a microbial bacterial agent for deodorizing domestic garbage and cultured excrement, a preparation method and an application. Background technique [0002] With the improvement of people's living standards, more and more domestic waste is produced, and the organic matter content in domestic waste is on the rise year by year. In large domestic cities, the organic matter content of domestic waste has exceeded 60%. During the transfer process, odorous gases such as ammonia and hydrogen sulfide are produced, which seriously damage people's physical and mental health and pollute the environment. In line with the improvement of people's living standards, there are more and more breeding enterprises. The animal manure produced by the breeding enterprises cannot be treated reasonably and effectively, which makes the breeding places smelly and pollutes the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/14C02F11/02B01D53/84B01D53/58B01D53/52C12R1/865C12R1/25C12R1/07C12R1/125C12R1/645
CPCB01D53/52B01D53/58B01D53/84B01D2251/95C02F11/02C12N1/14C12N1/18C12N1/20Y02A50/20Y02P20/59
Inventor 施军营杨小波何清玉薛方亮田倩
Owner BIOLAND ENVIRONMENTAL TECH GRP CORP
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