Cryptosporidium filtering rate testing method for domestic purified water dispenser

Abstract

Disclosed is a cryptosporidium filtering rate testing method for a domestic purified water dispenser. The method comprises the steps that a micrometer colloid fluorescent microsphere is adopted as a cryptosporidium simulant, by configuring the simulation concentration, the simulant is pumped into a water supply pipe in a detected water purifier set through a microscale quantitative metering pump, purified water generated by unit reverse osmosis membrane filtering is subjected to real-time flow metering and then passes through a detachable stainless steel filter membrane device with the aperture being 0.22 micrometer, the filter membrane is taken down after metering sampling and placed in a fluorescence-free glass panel, observation and counting are performed under an epifluorescence microscope, and the cryptosporidium removal rate is obtained through calculation through computer image recognition and dimension measurement. By adopting the biological risk-free cryptosporidium simulant, the water dispersibility is good, fluorescent silica gel particles obviously distinguished from the background under the optical condition can be obtained, observation determination and geometric dimension determination can be achieved through electronic image collection by computer image software on the basis of the epifluorescence microscope, the experimental process is convenient to implement and repeated, and data is reliable.

Description

technical field [0001] The invention relates to a biotechnological detection method, in particular to a method for detecting the cryptosporidium filtration rate of a domestic pure water machine. Background technique [0002] Cryptosporidium Tyzzer (Cryptosporidium Tyzzer, 1907) is a tiny coccidia parasite. Widely exist in a variety of vertebrates, the main one that parasitizes humans and most mammals is C.parvum, and the disease caused by Cryptosporidium is called cryptosporidiosis. Diarrhea is the main clinical manifestation of zoonotic protozoan disease. The worm mainly parasitizes in the brush border nasopharynx vacuoles of the epithelial cells of the small intestine. The proximal end of the jejunum is the site with the largest number of parasites, and severe cases can spread to the entire digestive tract. It can also parasitize organs such as the respiratory tract, lungs, tonsils, pancreas, gallbladder and bile ducts. [0003] The worms parasitic on the intestinal mu...

AI Technical Summary

Problems solved by technology

This pathogen is currently an important factor that threatens the safety of drinking water in my country. With the popularization and use of household water purification equipment, it is difficult to test and evaluate the filtration effect of cryptosporidium for household water purification units due to inspection methods and technical limitations. Popularize and develop