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Multi-PCR primer group for human paternity identification and detection method

A paternity identification and detection method technology, applied to part or all of the nucleotide sequence and detection field, can solve the problems of limited amount of fetal cell-free DNA, SNP genetic difference interference, result deviation and other problems, so as to eliminate the difference in the number of Reads distribution and reduce The effect of mutation interference and background noise reduction

Active Publication Date: 2017-09-29
GUANGDONG ASCENDAS GENOMICS TECH CO LTD
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Problems solved by technology

However, the amount of fetal cell-free DNA used for non-invasive identification is limited, and the distribution of SNP markers is numerous. The enrichment process easily leads to large differences in the number of reads (Reads) in the sequencing results. Large interference may easily reduce the accuracy of non-invasive testing results in early pregnancy

Method used

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  • Multi-PCR primer group for human paternity identification and detection method
  • Multi-PCR primer group for human paternity identification and detection method
  • Multi-PCR primer group for human paternity identification and detection method

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Embodiment Construction

[0043] The invention will be described in detail below in conjunction with specific embodiments. But it is not intended to limit the present invention.

[0044] Herein, the expression "above" includes the original number.

[0045] 1. Use the multiplex PCR primer set composed of part or all of the SNP genetic marker sites shown in Table 1 to perform human parentage detection, and the following are the specific steps.

[0046] a. DNA extraction

[0047] The pregnant woman samples in this experiment were whole blood collected with Ardent blood collection tubes, and the plasma was obtained by two-step centrifugation. The specific operation was as follows: 4°C, 1600g, centrifuged for 10min, and the supernatant was drawn into a new 2mL EP tube. The collected supernatant was then centrifuged at 16000g for 10min at 4°C, and the supernatant was also pipetted into a new 2mL EP tube for later use.

[0048] (1) Extract fetal cell-free DNA

[0049] 1) Take 2mL of plasma, equilibrate to...

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Abstract

Screening conditions of SNP sites in paternity identification detection are optimized, and a multi-PCR primer group formed by SNP genetic marker sites is built according to the screening conditions. Human paternity identification is carried out by adopting the primer group, so that the defects of non-invasive paternity identification in early pregnancy can be overcome, the accuracy is high, and the multi-PCR primer group can be applied to judicial expertise and forensic medicine study.

Description

technical field [0001] The invention relates to the field of medical detection, which uses a specific SNP marker combination to detect human parentage, and specifically relates to part or all of the nucleotide sequences and detection methods in Table 1. Background technique [0002] Parentage identification refers to the identification of the blood relationship between individuals through the detection of human genetic markers and the analysis of genetic laws. The current method used for paternity testing is DNA analysis. Due to the limitations of the earliest first-generation genetic marker-restriction fragment length polymorphism (RFLP), the detection process is time-consuming and laborious, and only one can be detected at a time. The degree of polymorphism is low, so the application in paternity testing is gradually reduced; since then, the typing technology based on the second-generation genetic marker-short tandem repeat sequence (STR) has become an important method for...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/6869C12Q1/6888C12Q2600/156C12Q2537/143C12Q2535/122
Inventor 田荣荣洪小龙杨呈勇
Owner GUANGDONG ASCENDAS GENOMICS TECH CO LTD
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