Method for synthesizing ZIF-8 nano-micro spheres through using fibroin induction

Abstract

The invention discloses a method for synthesizing ZIF-8 nano-micro spheres through using fibroin induction. The method comprises the following steps: slowly adding a fibroin solution with the concentration of 5-20 wt% into a 2-methylimidazole [HmIm] solution, uniformly mixing the fibroin solution and the 2-methylimidazole [HmIm] solution, slowly adding a zinc nitrate hexahydrate [Zn(NO3)2.6H2O] solution, carrying out a magnetic stirring reaction for 15 min to obtain a white emulsion; and centrifuging the white emulsion at 8000-10000 rpm for 10 min, removing the obtained supernatant, cleaning the obtained precipitate by using a 50% alcohol solution 2-3 times, and freeze-drying the cleaned precipitate to obtain the ZIF-8 nano-micro spheres. The fibroin / ZIF-8 nano-micro spheres are directly synthesized at room temperature by using water as a solvent, so the method has the advantages of shortening of the synthesis time, low energy consumption in the whole treatment process, high biological safety, low price, simplicity and convenience in operation, and no pollution to the environment.

Description

technical field [0001] The invention belongs to the field of synthetic polymer materials, in particular to a method for synthesizing ZIF-8 nanometer microspheres induced by silk protein. Background technique [0002] ZIF8 is a kind of zeolite imidazolate framework material, which belongs to the subclass of metal organic framework (MOF). The N atoms on the 2-methylimidazole ring coordinate to form a ZnN with a regular tetrahedral structure 4 Then use these clusters as nodes to connect through the imidazole rings on 2-methylimidazole to form a cage coordination compound with regular hexahedral crystal form. Compared with other inorganic materials, it has the advantages of high specific surface area, adjustable functional pore size, flexible chemical structure, high thermal stability and superhydrophobicity, so it is widely used in gas separation and storage, biocatalysis and induction and Drug delivery and other aspects have broad application prospects. [0003] Silk protei...

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Problems solved by technology

[0004] Both ZIF-8 and silk protein can be used as excellent biomedical materials, and studies have shown that proteins (such as ribonuclease A, human serum albumin (HSA), lipase, hemoglobin, lysozyme, trypsin, urease, etc. ) can induce the synthesis of ZIF-8, but there is no relevant report about the synthesis of silk protein / ZIF-8 nanospheres with biological functions. The present invention utilizes silk protein to induce the synthesis of silk protein / ZIF-8 nanospheres, which can further expand silk protein Applications as biomedical materials