Method for synthesizing ZIF-8 nano-micro spheres through using fibroin induction

A technology of silk protein and nano-microspheres, which is applied in the field of silk protein-induced synthesis of ZIF-8 nano-microspheres, achieving the effects of low energy consumption, high biological safety, and improved biocompatibility

Inactive Publication Date: 2017-10-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Both ZIF-8 and silk protein can be used as excellent biomedical materials, and studies have shown that proteins (such as ribonuclease A, human serum albumin (HSA), lipase, hemoglobin, lysozyme, trypsin, urease, etc. ) can induce the synthesis of ZIF-8, but there is no

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1

[0018] (1) Prepare 10% silk fibroin solution, prepare 1600mM HmIm and 44.44mM Zn(NO 3 ) 2 ·6H 2 O;

[0019] (2) Add 5mL of prepared HmIm to the beaker, add 10mg of silk fibroin solution under slow magnetic stirring, and finally slowly add 5mL of Zn(NO 3 ) 2 ·6H 2 O, magnetic stirring for 15 min, a white emulsion is obtained;

[0020] (3) Centrifuge the white emulsion (8000-10000rpm, 10min), wash the resulting precipitate with 50% alcohol for 2-3 times, and freeze-dry to obtain silk protein / ZIF-8 nanospheres.

Example Embodiment

[0021] Example 2

[0022] (1) Prepare 10% silk fibroin solution, prepare 1600mM HmIm and 44.44mM Zn(NO 3 ) 2 ·6H 2 O;

[0023] (2) Add 5mL of prepared Zn(NO 3 ) 2 ·6H 2 O, adjust to alkaline environment with NaOH (generally pH=8), add 10mg of silk fibroin solution under magnetic stirring conditions, and finally slowly add 5mL of HmIm, magnetically stir for 15min to obtain a white emulsion;

[0024] (3) Centrifuge the white emulsion (8000-10000rpm, 10min), wash the precipitate 2-3 times with 50% alcohol, and freeze-dry to obtain silk protein / ZIF-8 nanospheres.

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Abstract

The invention discloses a method for synthesizing ZIF-8 nano-micro spheres through using fibroin induction. The method comprises the following steps: slowly adding a fibroin solution with the concentration of 5-20 wt% into a 2-methylimidazole [HmIm] solution, uniformly mixing the fibroin solution and the 2-methylimidazole [HmIm] solution, slowly adding a zinc nitrate hexahydrate [Zn(NO3)2.6H2O] solution, carrying out a magnetic stirring reaction for 15 min to obtain a white emulsion; and centrifuging the white emulsion at 8000-10000 rpm for 10 min, removing the obtained supernatant, cleaning the obtained precipitate by using a 50% alcohol solution 2-3 times, and freeze-drying the cleaned precipitate to obtain the ZIF-8 nano-micro spheres. The fibroin/ZIF-8 nano-micro spheres are directly synthesized at room temperature by using water as a solvent, so the method has the advantages of shortening of the synthesis time, low energy consumption in the whole treatment process, high biological safety, low price, simplicity and convenience in operation, and no pollution to the environment.

Description

technical field [0001] The invention belongs to the field of synthetic polymer materials, in particular to a method for synthesizing ZIF-8 nanometer microspheres induced by silk protein. Background technique [0002] ZIF8 is a kind of zeolite imidazolate framework material, which belongs to the subclass of metal organic framework (MOF). The N atoms on the 2-methylimidazole ring coordinate to form a ZnN with a regular tetrahedral structure 4 Then use these clusters as nodes to connect through the imidazole rings on 2-methylimidazole to form a cage coordination compound with regular hexahedral crystal form. Compared with other inorganic materials, it has the advantages of high specific surface area, adjustable functional pore size, flexible chemical structure, high thermal stability and superhydrophobicity, so it is widely used in gas separation and storage, biocatalysis and induction and Drug delivery and other aspects have broad application prospects. [0003] Silk protei...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08L87/00C08G83/00C08J3/12
CPCC08L89/00C08G83/008C08J3/12C08J2387/00C08J2389/00C08J2487/00C08J2489/00C08L87/00C08L2203/02
Inventor 杨明英陈玉平周官山
Owner ZHEJIANG UNIV
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