Screening method of sustained-release materials for Monochamus ahernatus Hope semiochemicals

A technology of long-released long-term beetle and slow-release materials, applied in the fields of botanical equipment and methods, preservation of human or animal bodies, chemicals for biological control, etc.

Inactive Publication Date: 2017-10-20
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to aim at the defects of pesticide control, trap control and biological control on the market at present, and to provide a simple and environmentally friendly, easy to operate, economical and practical, insect-resistant and long-lasting insect-repellent semiochemical slow-release material screening method

Method used

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  • Screening method of sustained-release materials for Monochamus ahernatus Hope semiochemicals
  • Screening method of sustained-release materials for Monochamus ahernatus Hope semiochemicals
  • Screening method of sustained-release materials for Monochamus ahernatus Hope semiochemicals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Preparation: (+)-α-pinene, (-)-β-pinene, and 2-undecyloxy-1-ethanol are formulated into a mixed solution of Monochamus alternatus semiochemicals in a ratio of 9:1:1. Use a pipette gun to transfer the semiochemical mixture to a low-density polyethylene (LDPE) slow-release bottle, and add it to the mouth of the bottle.

[0018] Determination: 7 groups were set up for experiments, 2 groups were used for GC analysis of the mixture ratio, and the other 5 groups were used for weighing to calculate the loss. To simulate the state of placing lures in the wild, hang the carrier lures on an impact-type Monochamus alternatus trap. A 7820A gas chromatograph (gas chromatograph 7820A, Agilent Company) was used for analysis, and the chromatographic column was a DB-5MS non-polar capillary chromatographic column (30m×0.25mm×0.25μm). Analysis conditions: heating program: keep at 80°C for 1min, then rise to 230°C at 5°C / min, and keep for 5 minutes. Samples 1 and 2 of the sustained-relea...

Embodiment 2

[0021] Preparation: (+)-α-pinene, (-)-β-pinene, and 2-undecyloxy-1-ethanol are formulated into a mixed solution of Monochamus alternatus semiochemicals in a ratio of 9:1:1. Use a pipette gun to transfer the semiochemical mixture to a high-density polyethylene (HDPE) slow-release bottle, and add it to the mouth of the bottle.

[0022] Determination: 7 groups were set up for experiments, 2 groups were used for GC analysis of the mixture ratio, and the other 5 groups were used for weighing to calculate the loss. To simulate the state of placing lures in the wild, hang the carrier lures on an impact-type Monochamus alternatus trap. A 7820A gas chromatograph (gas chromatograph 7820A, Agilent Company) was used for analysis, and the chromatographic column was a DB-5MS non-polar capillary chromatographic column (30m×0.25mm×0.25μm). Analysis conditions: heating program: keep at 80°C for 1min, then rise to 230°C at 5°C / min, and keep for 5 minutes. Samples 1 and 2 of the sustained-rele...

Embodiment 3

[0025] Preparation: (+)-α-pinene, (-)-β-pinene, and 2-undecyloxy-1-ethanol are formulated into a mixed solution of Monochamus alternatus semiochemicals in a ratio of 9:1:1. Due to the small hole of the polyethylene tube, inject 2mL of the semiochemical mixture with the injection needle, and seal both ends with a lighter.

[0026]Determination: 7 groups were set up for experiments, 2 groups were used for GC analysis of the mixture ratio, and the other 5 groups were used for weighing to calculate the loss. To simulate the state of placing lures in the wild, hang the carrier lures on an impact-type Monochamus alternatus trap. A 7820A gas chromatograph (gas chromatograph 7820A, Agilent Company) was used for analysis, and the chromatographic column was a DB-5MS non-polar capillary chromatographic column (30m×0.25mm×0.25μm). Analysis conditions: heating program: keep at 80°C for 1min, then rise to 230°C at 5°C / min, and keep for 5 minutes. Samples 1 and 2 of the sustained-release c...

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Abstract

The invention relates to a screening method of sustained-release materials for Monochamus ahernatus Hope semiochemicals. With the sustained release rate and the ratio of attractant components taken into account, a weighing method and a gas chromatography analysis method are adopted for screening the sustained-release material for the Monochamus ahernatus Hope semiochemicals. A Monochamus ahernatus Hope semiochemical mixed liquid is prepared from (+)-alpha-pinene, (-)-beta-pinene and 2-undecyloxy-1-ethanol in a ratio of 9: 1: 1, and four kinds of sustained-release materials, namely, LDPE (low-density polyethylene), HDPE (high-density polyethylene), a PE (polyethylene) tube and a PP (polypropylene) bottle, are selected as lures. Experimental results show that the four kinds of sustained release materials have certain advantages and disadvantages in terms of sustained release effect. The screening method of the sustained-release materials for the Monochamus ahernatus Hope semiochemicals can provide technical reference for developing a more economical and efficient attractant for Monochamus ahernatus Hope.

Description

technical field [0001] The invention relates to a screening method for a slow-release material of monochamus monochamus semiochemical substances. Background technique [0002] Monochamus alternatus Hope (Monochamus alternatus Hope) is the main trunk-boring pest of pine trees in my country. It can also cause pine wilt and cause death when it occurs alone. It is also the main vector insect of pine wood nematode, a devastating disease of pine trees. During the spread and infection of pine xylophilus, Monochamus alternata plays a key role in carrying, spreading and assisting the pathogen to invade the host. Therefore, effective control of the population and growth trend of Monochamus alternatus will help to protect economic pine forests and landscape pine forests. [0003] At present, the control of monochamus larvae is mainly based on poisoning, mainly including fumigation of diseased and dead wood and soaking in chemicals. This method is generally time-consuming and laborious...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/10A01N25/18A01N27/00A01N31/02A01P19/00A01M1/02
CPCA01M1/023A01M1/026A01N25/10A01N25/18A01N27/00A01N31/02A01N2300/00
Inventor 马涛蓝来娇王偲孙朝辉王忠茅裕婷朱诚棋温秀军
Owner SOUTH CHINA AGRI UNIV
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