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Method for preparing natural antioxidant from clam worm

A technology of natural antioxidants and manufacturing methods, applied in the fields of antitoxins, food science, food preservation, etc., can solve the problems of methods and related reports of antioxidant products without an antioxidant system, and achieve good research and application prospects, preservation and other problems. The effect of stable, good antioxidant activity

Inactive Publication Date: 2017-10-20
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The literature also reported a set of anti-oxidation system in the clam worm body. It was reported that the clam worm can prevent the poisoning of lead ions very well and has a good antioxidant function, but the article did not report how to prepare the antioxidant system into an anti-oxidation system. Methods and related reports of oxidizing products

Method used

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  • Method for preparing natural antioxidant from clam worm
  • Method for preparing natural antioxidant from clam worm

Examples

Experimental program
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Effect test

Embodiment 1

[0020] The Nereis japonica was placed in a damp environment of seawater for 1 day, and the seawater was temporarily raised for 1 day. Take 1 part of clamworm, wash it with deionized water, and remove surface impurities and blood. Homogenize at 5,000 rpm for 15 minutes after shearing the clamworm individual, and ultrasonically break it for 10 minutes. Wash and soak 30 parts of deionized water overnight, decoct for 1 h, add 15 parts of deionized water after cooling and continue to boil for 20 min, mix the two decoctions, cool and stand at 4 °C for 5 h, concentrate and freeze-dry. Promptly obtain the clam worm antioxidant product, the product is brown viscous, and the yield is 13.63%.

Embodiment 2

[0022] The clam worms were placed in a humid seawater environment for 7 days, and then temporarily raised in seawater for 3 days. Take 1 part of clamworm, wash it with deionized water, and remove surface impurities and blood. Homogenize at 10,000 rpm for 25 min and ultrasonically break for 20 min. Wash and soak 30 parts of deionized water overnight, decoct for 1 h, add 15 parts of deionized water after cooling and continue to boil for 20 min, mix the two decoctions, cool and stand at 4 °C for 5 h, concentrate and freeze-dry. Promptly obtain the clam worm antioxidant product, the product is reddish-brown viscous, and the yield is 10.80%.

Embodiment 3

[0024] The clam worm was placed in the seawater damp environment for 3 days, and the seawater was temporarily raised for 2 days. Take 1 part of clamworm, wash it with deionized water, and remove surface impurities and blood. Homogenize at 5,000 rpm for 15 minutes after shearing the clamworm individual, and ultrasonically break it for 10 minutes. Wash and soak 30 parts of deionized water overnight, decoct for 1 h, add 15 parts of deionized water after cooling and continue to boil for 20 min, mix the two decoctions, cool and stand at 4 °C for 5 h, concentrate and freeze-dry. Promptly obtain the clam worm antioxidant product, the product is reddish-brown viscous, and the yield is 11.68%.

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Abstract

The invention discloses a method for preparing a natural antioxidant from clam worm. The method comprises the following steps: taking the clam worm as a raw material, storing and treating the clam worm in a sheltered and wet seawater environment, flushing with deionized water, homogenizing, performing ultrasonic treatment, soaking overnight, and boiling until the clam worm skeleton is ivory; filtering to remove impurities, concentrating, and performing freeze drying, thereby obtaining the product. The aqueous soaking extraction, boiling separation, ultrafiltration membrane concentration and freeze drying technologies are adopted, no other chemical reagent is used, the operating process is simple, the process stability is excellent, and the extraction rate is high. The anti-oxidation product manufactured by the invention is stable to preserve, has excellent antioxidant activity and is capable of clearly scavenging free radicals such as superoxide anions and the like. Therefore, the product has good research and application prospects in the aspects such as medicines, health care and the like.

Description

technical field [0001] The invention belongs to the field of biological products and relates to a method for preparing natural antioxidants from clam worms. Background technique [0002] In taxonomy, clam worms belong to the phylum Annelida, polychaetes, wandering orders, and nereisidae, commonly known as sea worms, sea maggots, sea centipedes, and sea leeches. There are more than 80 kinds of clamworms in my country, and the economic species and varieties used for breeding mainly include: Japanese clamworm, spiny clamworm, double-tooth clamworm, etc. It likes to live in coastal beaches with fresh water inflow, and in the sand and mud of the middle intertidal zone to the subtidal zone. The larvae eat plankton, and the adults feed on humus. Lugworm has high nutritional value and is widely used in aquaculture. Nereid (Nereid), the English name—Clamworm, belongs to the phylum Annelids, a family of Polychaete Phyllophthora order, and has high economic value. [0003] The cla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3472A61K35/64A61P39/06
CPCA23L3/3472A61K35/63
Inventor 陈西广周忠政程晓杰孔明刘雅冯超
Owner OCEAN UNIV OF CHINA