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Method for promoting hematopoietic stem cell homing and implantation

A technology of hematopoietic stem cells and drugs, applied in extracellular fluid diseases, blood diseases, pharmaceutical formulations, etc., can solve problems to be further studied, and achieve the effect of promoting homing and implantation

Active Publication Date: 2017-10-24
ACADEMY OF MILITARY MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the related function of caffeic acid phenethyl ester (CAPE) remains to be further studied

Method used

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  • Method for promoting hematopoietic stem cell homing and implantation
  • Method for promoting hematopoietic stem cell homing and implantation
  • Method for promoting hematopoietic stem cell homing and implantation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Follow the steps below to detect the effect of phenethyl caffeate on the survival rate of mice after irradiation myeloablation and bone marrow transplantation:

[0042] 1. Isolation of mouse bone marrow cells

[0043] C57BL / 6J strain mice (this strain of mouse hematopoietic stem cells express CD45.2 markers, which can be detected and identified by flow cytometry) were purchased from Beijing Weitong Lihua Company, male, 5 weeks old, weighing 16-18 g. After more than one week of steady state, mice aged 6-8 weeks were selected for experiments.

[0044] The mice were killed by spinal dislocation, the femur and tibia were taken out, and the bone marrow cavity was washed repeatedly with PBS containing 2% fetal bovine serum (Gibco). The obtained cell suspension was centrifuged at 2000rpm for 5 minutes, the supernatant was discarded, and the cell pellet was resuspended in PBS. Use mouse peripheral blood lymphocyte separation medium (purchased from Haoyang Biological Joint Labo...

Embodiment 2

[0052] Follow the steps below to detect the effect of phenethyl caffeate on promoting the homing of mouse hematopoietic stem cells:

[0053] 1. Transplantation after irradiated myeloablation and intraperitoneal injection of the small molecule compound phenethyl caffeate

[0054] After one week of steady state of C57BL / 6J strain mice, select mice with age (6-8 weeks) and body weight (20-22g), and randomly divide them into 3 groups, with 4 mice in each group as recipients, respectively labeled as blank group , control group and experimental group were irradiated with Co60 at a dose of 9.5Gy. Within 18-20 hours after irradiation, the same batch of mice was used as the provider of hematopoietic stem cells, and fresh bone marrow cell suspension was prepared according to the method in Example 1, and the tail veins of each mouse in the control group and experimental group were transplanted with 1× 10 7 cells. 20-24 hours before irradiation, 0 hours after irradiation and 0 hours af...

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Abstract

The invention provides a method for promoting hematopoietic stem cell homing and implantation, a purpose of caffeic acid phenethyl ester to medicine preparation, a kit for promoting hematopoietic stem cell homing and implantation, and a medicine composition. According to the embodiment, the medicine composition using the caffeic acid phenethyl ester or pharmaceutically acceptable salts of the caffeic acid phenethyl ester as active ingredients has the obvious effect of promoting hematopoietic stem cell homing and implantation.

Description

technical field [0001] The present invention relates to the field of biology, in particular, the present invention relates to a method for promoting homing and implantation of hematopoietic stem cells, the use of phenethyl caffeate in the preparation of medicines, a kit for promoting homing and implantation of hematopoietic stem cells, and a pharmaceutical composition . Background technique [0002] Hematopoietic stem cell transplantation has been clinically used to treat malignant tumors of the blood and lymphatic system for decades, but the success rate of this treatment is only 40-70%. After the hematopoietic stem cells transplanted from the donor into the recipient, they need to go through the blood circulation, cross the bone marrow microvascular endothelial cells, migrate down, homing to the bone marrow microenvironment, adapt to the bone marrow microenvironment, re-proliferate, and finally re-enter the recipient's blood circulation , in order to achieve the purpose o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/216A61P7/00A61P35/00
CPCA61K31/216
Inventor 裴雪涛李艳华陈小芳张博文何丽娟
Owner ACADEMY OF MILITARY MEDICAL SCI
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