A new use of Digi and an antitumor drug
A drug and action technology, applied in the field of antitumor drug preparation, can solve the problems of insufficient research, unclear active ingredients, unclear active sites, and unclear mechanism of action, and achieve excellent clinical application prospects, no toxic side effects, and effects. Mechanistically clear effect
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Embodiment 1
[0032] The real-time quantitative gene amplification fluorescence detection system, that is, the qPCR method, was used to verify the effect of the grass extract on the expression of NFKB2, EGFR, and WNT7B genes. The specific experimental process was as follows:
[0033] 1. Weigh 10g of Di whole grass, add 150mL of 90% ethanol and heat to 85°C, extract for 3h, concentrate and dry to obtain the extract of Di grass. Dissolve the grass extract in DMSO, and treat the cells for 24 hours;
[0034] 2. Use the kit to extract RNA and reverse it to cDNA;
[0035] 3. RT-qPCR reaction, program: 95°C, 3min; (95°C, 3s; 60°C, 30s) 40 cycles.
[0036] Among them, the primer sequence is:
[0037] NFKB2: Upstream primer AGAGGCTTCCGATTTCGATATGG
[0038] Downstream primer GGATAGGTCTTTCGGCCCTTC
[0039] EGFR: Upstream primer AGGCACGAGTAACAAGCTCAC
[0040] Downstream primer ATGAGGACATAACCAGCCACC
[0041] WNT7B: Upstream primer GAAGCAGGGCTACTACAACCA
[0042] Downstream primer CGGCCTCATTGTTATGC...
Embodiment 2
[0049] Using the CCK8 experiment to detect the effect of Di (whole grass) extract on cell proliferation, the specific experimental process is as follows:
[0050] 1. Treat breast cancer MDA-MB-231 cells and breast cancer MCF7 cells for 24 hours with the extract of Di (whole plant) respectively;
[0051] 2. Use CCK8 kit to measure cell viability;
[0052] 3. Calculation of cell viability, inhibition rate, IC 30 , IC 50 .
[0053] The results of CCK8 are shown in Table 2, Table 3 and figure 2 , image 3 .
[0054] Table 2 The effect of Di (whole plant) on the proliferation of MDA-MB-231 cells
[0055]
[0056]Table 3 Effect of Di (whole plant) on the proliferation of MCF7 cells
[0057]
[0058] The results of CCK8 showed that the extract concentration of Di (whole grass) could inhibit the activity of tumor cells in the range of 50-400 μg / mL. IC 30 The value is 160μg / mL, IC 50 The value is about 250μg / mL; IC for MCF7 cells 30 The value is 98μg / mL, IC 50 The va...
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