Blood cell capture chip and method
A blood cell and chip technology, applied in the field of biomedicine, can solve the problems of being unable to be distinguished, reducing the detection efficiency, and not being able to effectively collect tumor cells, etc., and achieve the effect of effective enrichment and differentiation
Pending Publication Date: 2017-10-24
CHINA COMM TECH CO LTD +1
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[0003] However, the technology of separating tumor cells by membrane filtration cannot effectively collect tumor cells of smaller size. The density gradient centrifugation method will cause the separated tumor cells to mix with white blood cells and monocytes in the blood sample, which reduces the detection efficiency. Magnetic bead technology results in a small number of indistinguishable non-specifically bound cells in isolated tumor cells
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[0026] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are the embodiment of the present invention. Some examples, but not all examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.
[0027] The terms "comprising" and any variations thereof in the description and claims of the present invention and the above drawings are intended to cover non-exclusive inclusion. For example, a process, method or system, product or device comprising a series of steps or units is not limited to the listed steps or units, but optionally also inc...
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An embodiment of the invention provides a blood cell capture chip and method. Specific antibody-fluorescent molecule-magnetic nanoparticles are arranged in a micro-channel, and a magnetic structure is fixed at the bottom of a glass substrate, so that the specific antibody-fluorescent molecule-magnetic nanoparticles are attracted and fixed by the magnetic structure, target cell antigen in blood is captured by the specific antibody-fluorescent molecule-magnetic nanoparticles, fluorescent molecules in the specific antibody-fluorescent molecule-magnetic nanoparticles emit fluorescence due to the fact that the fluorescent molecules gather to be in a stimulated state, effective enrichment and distinguish of the target cell antigen are realized, and the blood cell capture chip and method can be widely applied to detection operation of pathological cells such as circulating tumor cells, cancer cells and the like.
Description
technical field [0001] The embodiments of the present invention belong to the technical field of biomedicine, and in particular relate to a blood cell capture chip and method. Background technique [0002] In the field of biomedical technology, it usually involves detection of some diseased cells in blood. Taking circulating tumor cells (circulating tumor cells, CTCs) as an example, before detecting and diagnosing tumor cells, it is usually necessary to separate the tumor cells. Commonly used tumor cell separation techniques include membrane filtration separation of tumor cells (Isolation by size of epithelial tumor cells, ISET), density gradient centrifugation (density gradient centrifuge), and immunomagnetic bead technology (immunomagnetic bead, IMB). [0003] However, the technology of separating tumor cells by membrane filtration cannot effectively collect tumor cells of smaller size. The density gradient centrifugation method will cause the separated tumor cells to mix...
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Login to View More IPC IPC(8): G01N33/569G01N33/533
CPCG01N33/533G01N33/56966
Inventor 韩琳丁庆刘荣跃李辰
Owner CHINA COMM TECH CO LTD