Preparation method of stationary phase of high performance liquid chromatography and chromatographic column
A high-performance liquid chromatography and stationary phase technology, applied in the field of high-performance liquid chromatography stationary phase preparation methods and chromatographic columns, can solve problems such as inability to achieve complete separation, inability to completely separate natural product analyte analysis, and achieve excellent selectivity, The preparation method is simple and the effect of broad application prospects
Inactive Publication Date: 2017-11-03
SOUTHWEST UNIV
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[0003] In summary, the existing problems in the prior art are: the existing commercial stationary phases often cannot achieve complete separation when separating structural analogues, and there are gaps that cannot be completely separated when analyzing analytes with complex components such as natural products. difficulty
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[0024] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0025] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.
[0026] like figure 1 As shown, the high performance liquid chromatography stationary phase preparation method provided by the embodiments of the present invention comprises the following steps:
[0027] S101: Synthesis of functional group 4-(2-phenylchromanone) methacrylate;
[0028] S102: Preparation of mercaptopropyltrimethoxysilane modified silica gel;
[0029] S103: Preparation of stationary phase based on mercaptoalkene click chemistry method;
[0030] S104: Prepare the statio...
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The invention belongs to the technical field of high-performance liquid-phase chromatographic columns and discloses a preparation method of a stationary phase of a high performance liquid chromatography and a chromatographic column. The preparation method comprises the following steps: synthesizing a functional group 4-(2-phenylchromone)methacrylate; preparing mercaptopropyltrimethoxysilane modified silica gel; preparing the stationary phase based on a thiololefin click chemical method; taking methanol as a homogenization agent to prepare homogenized slurry of the stationary phase; taking the methanol as displacement fluid and adding the methanol into a 150mm*4.6mm (i.d.) chromatographic column under the pressure of 40MPa; washing the filled chromatographic column with pure methanol for 30 times of column volumes at a flow speed of 0.3ml / min. The column efficiency of the high-performance liquid-phase chromatographic column prepared by the preparation method is equivalent to the column efficiency of a commercial chromatographic column; the preparation method is simple and convenient and has a wide application prospect.
Description
technical field [0001] The invention belongs to the technical field of high-performance liquid chromatography columns, and in particular relates to a preparation method of a high-performance liquid chromatography stationary phase and a chromatography column. Background technique [0002] High-performance liquid chromatography column is known as the heart of high-performance liquid chromatography, which is the basis for realizing multi-component separation and analysis. At present, the chromatographic column using C18 or C8 as the bonded phase is widely used in high-performance liquid chromatography analysis. The separation mechanism mainly depends on its hydrophobic effect. Different ratios are used to achieve the separation of multiple components. However, when this type of stationary phase separates targets with similar hydrophobicity or polarity, ideal separation results cannot be obtained in many cases. However, in many cases, the actual analysis work requires the stat...
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IPC IPC(8): B01J20/281B01J20/30B01D15/22G01N30/60
CPCB01D15/22B01J20/281B01J20/30G01N30/60
Inventor 彭焕军彭敬东
Owner SOUTHWEST UNIV