Method for extracting 1-deoxynojirimycin from silkworm excrement

Abstract

The invention discloses a method for extracting 1-deoxynojirimycin from silkworm excrement. The method is characterized by comprising the following steps: (1) carrying out continuous countercurrent extraction on clean silkworm excrement, so as to obtain a silkworm excrement extract liquid and a paste mixture; (2) carrying out flocculation and sedimentation on the extract liquid to obtain a supernatant; (3) carrying out ultrafiltration and impurity removal on the supernatant to obtain an ultrafiltration permeate liquid; (4) nano-filtering and concentrating the ultrafiltration permeate to obtain a nano-filtration concentrate; (5) carrying out extraction and separation on the nano-filtration concentrate to obtain an extraction liquid; and (6) concentrating and drying the extraction solution to obtain a finished product containing 1-deoxynojirimycin, wherein the mass fraction of 1-deoxynojirimycin accounts for 20-30% of the finished product. The method is easy and convenient to operate, low in cost, capable of saving energy, protecting environment, and suitable for industrial production.

Description

technical field [0001] The invention relates to a technology for extracting pharmacological active ingredients from natural products, in particular to a method for extracting 1-deoxynojirimycin from silkworm excrement. Background technique [0002] 1-Deoxynojirimycin (DNJ), is a polyhydroxy natural alkaloid present in mulberry leaves and silkworm excrement, with a content of about 0.1%, and a relatively high content in known plants. Its molecular formula is C 6 h 13 NO 4 , with a molecular weight of 163.17, is currently the only internationally recognized zero-harm biological agent in the prevention and treatment of diabetes market, and has broad application prospects in the fields of medicine, health products, and functional foods. [0003] At present, there are mainly the following methods for extracting 1-deoxynojirimycin in China: 1. Extract the mulberry leaves after pulverization, acidify with inorganic acid, then pass through cation exchange resin, elute with ammonia...

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Problems solved by technology

[0003] At present, there are mainly the following methods for extracting 1-deoxynojirimycin in China: 1. Extract the mulberry leaves after pulverization, acidify with inorganic acid, then pass through cation exchange resin, elute with ammonia water, concentrate and dry, and obtain DNJ product. Although the process is simple, but through the cation exchange resin, the loss is relatively large

2. The mulberry leaves are extracted, concentrated, microporous filtered, filtered through an organic ultrafiltration membrane, then passed through a macroporous adsorption resin and a cation exchange resin, and finally concentrated and dried to obtain a product with a purity of more than 50%, but the process is cumbersome , complex process, large investment in equipment, unfavorable for industrial production

3. Using mulberry leaves as raw materials, after pretreatment, enzymatic hydrolysis, filtration, continuous combined extraction (tank batch batch type), concentration, alcohol precipitation, centrifugation, membrane filtration, concentration, and drying, this method has cumbersome processes, complicated processes, Defects such as high cost

[0004] In summary, the current extraction method has the following problems. One is to use mulberry leaves as a raw material, which competes with silkworms for food, so the resource utilization rate is low and the cost of raw materials is high. Time-consuming, polluting the environment, not conducive to industrial production

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