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Culture medium series and tissue culture method for quickly breeding nandina domestica fire power

A technology of flame Nandina and multiplication medium, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low rooting rate, long cultivation period, etc., and achieve strong seedling resistance, short cultivation period, The effect of high multiplication coefficient

Inactive Publication Date: 2017-11-10
江苏丰收大地种业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few domestic research reports on flame nandina, and most of them have problems such as long cultivation period and low rooting rate.

Method used

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  • Culture medium series and tissue culture method for quickly breeding nandina domestica fire power

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Acquisition and sterilization of explants: remove pest-free Nandina stem tips from the Nandina plant, soak them in 75% alcohol for 30 seconds, and then soak them in mercuric chloride with a molar concentration of 0.1% 8min.

[0024] Germination culture: inoculate the sterilized stem tip of Nandina sativa onto the sterilized germination medium, and cultivate it for 20-30 days to obtain tissue cultured seedlings of Nandina lanceolata. The cultivation environment of the germination cultivation is as follows: the light intensity is 1800-2000Lx, the light time is 14d / h, and the culture temperature is 24-26°C. The formula of the germination medium is: MS+6-BA0.2mg / L+sucrose 30g / L+agar 5.5g / L, and the pH value is 5.8-6.0.

[0025] Proliferation culture: remove the root hairs of the flame nandina tissue culture seedlings, inoculate them on the sterilized proliferation medium, and cultivate them for 25-35 days. The culture environment for the proliferation culture is: light in...

Embodiment 2

[0028] Acquisition and sterilization of explants: remove pest-free Nandina stem tips from the Nandina plant, soak them in 75% alcohol for 30 seconds, and then soak them in mercuric chloride with a molar concentration of 0.1% 8min.

[0029] Germination culture: inoculate the sterilized stem tip of Nandina sativa onto the sterilized germination medium, and cultivate it for 20-30 days to obtain tissue cultured seedlings of Nandina lanceolata. The cultivation environment of the germination cultivation is as follows: the light intensity is 1800-2000Lx, the light time is 14d / h, and the culture temperature is 24-26°C. The formula of the germination medium is: MS+6-BA0.5mg / L+sucrose 20g / L+agar 5.5g / L, and the pH value is 5.8-6.0.

[0030] Proliferation culture: remove the root hairs of the flame nandina tissue culture seedlings, inoculate them on the sterilized proliferation medium, and cultivate them for 25-35 days. The culture environment for the proliferation culture is: light in...

Embodiment 3

[0033] Acquisition and sterilization of explants: remove pest-free Nandina stem tips from the Nandina plant, soak them in 75% alcohol for 30 seconds, and then soak them in mercuric chloride with a molar concentration of 0.1% 8min.

[0034] Germination culture: inoculate the sterilized stem tip of Nandina sativa onto the sterilized germination medium, and cultivate it for 20-30 days to obtain tissue cultured seedlings of Nandina lanceolata. The cultivation environment of the germination cultivation is as follows: the light intensity is 1800-2000Lx, the light time is 14d / h, and the culture temperature is 24-26°C. The formula of the germination medium is: MS+6-BA0.5mg / L+sucrose 40g / L+agar 5.5g / L, and the pH value is 5.8-6.0.

[0035] Proliferation culture: remove the root hairs of the flame nandina tissue culture seedlings, inoculate them on the sterilized proliferation medium, and cultivate them for 25-35 days. The culture environment for the proliferation culture is: light in...

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Abstract

The invention discloses a culture medium series and tissue culture method for quickly breeding nandina domestica fire power. The culture medium series comprises a germination culture medium, a propagation culture medium and a rooting culture medium, wherein compositions and content of each component of the culture medium series are respectively as follows: the germination culture medium: MS+6-BA0-0.5mg / L+sucrose20-40g / L; the propagation culture medium: MS+6-BA0.5-1.5mg / L+NAA0.05-0.2mg / L+sucrose20-40g / L; the rooting culture medium: 1 / 2MS+IBA0.1-0.5mg / L+sucrose10-30g / L. When being used for tissue culture of nandina domestica fire power seedlings, the culture medium series has the characteristics of short culture period, high propagation coefficient, good rooting effect, high seedling resistance and the like; with adoption of a tissue culture-based vegetative propagation technology, not only can good characters of female parents be kept, but also high-quality seedlings can be obtained; the used raw materials for the culture media can be easily purchased from the market, and the culture media are easy to prepare.

Description

technical field [0001] The invention relates to a plant seedling production method, in particular to a flame nandina rapid propagation medium series and a tissue culture method, belonging to the field of biotechnology. Background technique [0002] Flame Nandina belongs to the genus Nandina of Berberidaceae. The plant itself is short, the stems are short, and the leaves are oval or ovate. Flame nandina is one of the most popular color-leaved tree species in landscaping at present, with great economic and ornamental value. [0003] The commonly used propagation methods of flame nandina are branch propagation and cutting propagation. These two propagation methods have the defects of slow propagation speed and degraded variety characteristics. In order to break through the technical bottleneck in the propagation of Nandina flavescens, in recent years people have begun to seek rapid propagation methods of Nandina flamingus, and tissue culture is one of the methods. At present,...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈明亮张丽丽周志疆张荣梅杨佩娟
Owner 江苏丰收大地种业发展有限公司
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