Co-culture device

Abstract

The invention discloses a co-culture device. The co-culture device comprises a dish body, a dish cover, a bracket, small dishes and culture films. The top surface of the dish body is open. The dish cover covers the opening of the dish body. The bracket supports in the dish body. The bracket is provided with clamping positions fitting to the small dishes in an array way. The small dishes are clamped in the clamping positions. The bottom surface of each one of the small dishes is open and the culture films are compounded to the openings of the small dishes. The culture film is treated by plasma. The co-culture device can culture a variety of cells in the same environment and the cells contact and interact with each other so that the microenvironment similar to the internal environment is simulated and the problem that the single-layer culture method cannot provide a normal tissue growth environment and the problems of inconsistent culture environments and time and labor waste are solved.

Description

technical field [0001] The invention relates to a cell culture device, in particular to a co-culture device. Background technique [0002] In the late 1980s, in order to establish a culture system more similar to the in vivo environment and make the in vitro environment as consistent as possible with the in vivo environment, so that cells can communicate with each other and support each other for growth and proliferation, people based on cell culture technology Cell co-culture technology has been developed. Cell co-cultivation technology is to co-cultivate two or more types of cells in the same environment. Because it has the advantage of better reflecting the in vivo environment, this method is widely used in modern cell research. [0003] Currently, cell co-culture technology is mostly used in bone cells and nerve cells. The cell co-culture system is mainly established by two methods: 1) direct co-culture system, that is, two or more types of cells are seeded in the same...

AI Technical Summary

Problems solved by technology

[0005] 1) The conventional in vitro monolayer culture method cannot provide the environmental conditions required for the normal growth and development of tissues. The genome of each cell in the body is the same, but its phenotype and functional structure are different in different organs and tissues. In many cases, monolayer Layered cell culture will gradually lose its original biological characteristics due to changes in the in vitro environment, and there will be completely different experimental results in which the effect of the outside world on the body

[0006] 2) Tissues and organs contain a variety of cells, such as observing the interaction between two cells will take a lot of time, energy and expense, and require multiple cultures of various cells, and the culture conditions are different

[0007] 3) At present, most of the in vivo experiments are based on animal experiments, but the overall animal experiments take a long time, the experimental results are unstable, and the repeatability is low. Raising animals consumes a lot of energy and financial resources, which is not convenient for the detection and observation of the experimental process. The number and types of animals are limited, and it is difficult for animal experiments to reflect the sensitivity of real clinical patients to drugs. At the same time, animal experiments are against humanitarianism

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