A kind of cell transplantation synergistic protection device and preparation method thereof

A technology of cell transplantation and chloroform solution is applied in the field of medical transplantation device and its preparation, which can solve the problems of difficult fixation of transplanted cells, lack of oxygen and nutrients at the transplantation site, and achieves the effects of safe and effective regulation, simple structure and easy processing.

Active Publication Date: 2019-03-26
HUNAN XENO LIFE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the current immune rejection of cell transplantation, the lack of oxygen and nutrients in the transplantation site, and the difficulty of fixing the transplanted cells in the target site to play related roles, we use high biocompatibility materials as raw materials to develop a new type of cell transplantation synergistic Protective device and method for its preparation, facilitating fixation, long-term survival, augmentation and removal of cell-specific site transplantation

Method used

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  • A kind of cell transplantation synergistic protection device and preparation method thereof
  • A kind of cell transplantation synergistic protection device and preparation method thereof
  • A kind of cell transplantation synergistic protection device and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 The preparation of the transplantation synergistic device of the present invention uses:

[0026] Dissolve 4g of poly DL-lactide-caprolactone in 50ml of chloroform, add 20g of NaCl and stir thoroughly, then transfer to a sterile petri dish, wait for the chloroform to dry up in an ultra-clean workbench to obtain a transplant piece, rinse the transplant with sterile distilled water piece. The transplant block is cast into 10×15×5mm, with a volume of 750mm 3 . A hollow iron pipe with a hole diameter of 0.4 mm is used to punch holes on the side of the stent to make a graft storage pipeline, and then a hydrophobic polyethylene tube is used to insert the graft storage pipeline. Add 2mg human fibrinogen, 50ng vascular endothelial growth factor, 100ng epidermal growth factor, 2ul 1% penicillin-streptomycin and 20ul thrombin in 2ml CMRL medium to form a fibrin gel, soak the transplantation device in fibrin In the gel, the fibrin gel is solidified within the micropor...

Embodiment 2

[0028] Example 2 Transplantation of pancreatic islet cells in diabetic model mice

[0029] The first group of diabetic model mice were subcutaneously transplanted into the transplantation device of the present invention on their backs and waists. After 4 weeks, after vascularization in the transplantation device, a small incision was made on the top of the mouse back transplantation device, and the hydrophobic polyethylene tube was taken out. To provide a channel for islet cells, inject 1200 islet cells into the channel with a 23G Hamilton syringe, then seal the port of the channel with poly-DL-lactide-caprolactone, and suture the incision. The second group of diabetic model mice were directly transplanted with 1200 islet cells under the renal capsule. The third group of diabetic model mice was not transplanted with any islet cells as a control.

Embodiment 3

[0030] Example 3 Detection of Blood Glucose in Diabetic Model Mice

[0031] After islet cell transplantation, the diabetic model mice in the first, second and third groups were tested for blood glucose levels every week until the tenth week, and the diabetic model mice in the third group were used as controls. Experimental results show that after using the device of the present invention to transplant islet cells, the blood sugar of diabetic mice drops significantly, and the blood sugar can maintain a stable level within 10 weeks. The blood sugar of the diabetic mice in the kidney capsule transplantation group decreased significantly at the initial stage and remained stable, but the blood sugar level gradually increased and the fluctuation gradually became larger in the later stage. The blood sugar of the diabetic mice in the non-transplanted group maintained a high blood sugar level all the time, and the blood sugar fluctuated greatly.

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Abstract

The invention belongs to the technical field of medical transplantation devices and preparation of the medical transplantation devices, and in particular relates to a cell transplantation synergistic device and a preparation method thereof. The cell transplantation synergistic protective device comprises transplant blocks made of highly biocompatible GMP-grade materials, namely poly(DL-lactide-caprolactone), and cell storage pipelines, as well as angiogenesis related factors. The cell transplantation device can carry any treatment human cells to be transplanted to subcutaneous, muscle and abdominal parts, renal cysts and specific treatment parts. In addition, the transplantation device can promote the formation of blood vessels around storage tubes of grafts, so that the grafts can play a role in transplantation parts, and secreted therapeutic substances further can reach the whole body through peripheral blood vessels to play related curative effects. The transplantation device can increase or remove the grafts in the process of transplantation properly; therefore, the dose of the grafts can be easily, fast, safely and effectively regulated. The cell transplantation synergistic protective device disclosed by the invention is simple in structure, easy to manufacture and capable of realizing large-scale production, and plays a role of treating related diseases.

Description

technical field [0001] The invention belongs to the technical field of medical transplantation devices and its preparation, and relates to a transplantation treatment synergistic protection device suitable for various cells and its preparation technology, in particular to a pancreatic islet cell transplantation synergistic protection device and its preparation technology. Background technique [0002] With the development and development of the medical field, a new type of treatment has appeared in people's field of vision. Cell transplantation utilizes the characteristics of certain cells with specific functions, which are obtained by bioengineering methods or processed through in vitro expansion and special culture, and have therapeutic effects such as promoting tissue and organ regeneration and collective rehabilitation to achieve the purpose of treating certain diseases. This method has also attracted more and more attention from scientists. [0003] Nowadays, cell tran...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/06A61L31/04A61L31/14A61L31/16
CPCA61L31/046A61L31/06A61L31/14A61L31/146A61L31/16A61L2300/254A61L2300/406A61L2300/414C08L67/04
Inventor 王维易受南蒋健晖武明花
Owner HUNAN XENO LIFE SCI
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