Method for extracting plant anthocyanins

An anthocyanin and plant technology, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of uncoordinated solvent use process, difficult separation and purification, complicated preparation steps, etc., and achieve 100% atom utilization , Improve the extraction efficiency, the effect of cheap and easy-to-get raw materials

Active Publication Date: 2019-11-26
黑龙江天阔源饮品有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex preparation steps, low extraction efficiency, large differences in the organic solvents used by anthocyanins of different polarities, and solvent toxicity and volatility, it is very easy to cause environmental pollution and organic solvent residues in the extract.
[0004] The emergence of ionic liquids was once considered to be a good substitute for traditional organic solvents, but with the deepening of research, people gradually found that these solvents were in some irreconcilable contradictions in the process of use.
For example, ionic liquids generally have high viscosity, recovery and separation and purification of products are difficult; and the preparation process of most ionic liquids is not green, and their own separation and purification are difficult, water absorption is serious, and the preparation cost is high; traditional imidazole and pyridine ionic liquids It is non-renewable, difficult to degrade, and has obvious toxicity, etc., which directly leads to difficulties in the industrial application of ionic liquids.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting plant anthocyanins
  • Method for extracting plant anthocyanins
  • Method for extracting plant anthocyanins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0027] This embodiment provides a method for extracting anthocyanins from mulberries. Take fresh mulberry fruits, remove the stems, beat them into a homogenate with a mixer, take 10 g of the homogenate and use the deep eutectic solvent choline chloride-lactic acid (molar ratio 1: 3, water content 0%wt) as the extraction solvent, extract for 10 minutes under the conditions of solid-to-liquid ratio 1g: 30mL, ultrasonic power 100W, electrostatic field strength 4kV, extraction temperature 50°C to obtain anthocyanin extract; add the above anthocyanin extract Add 5g of attapulgite-loaded chitosan cross-linked cyclodextrin complex, shake it on a shaking table at 30°C and 100r / min for 4 hours, take the precipitate after centrifugation, wash it with deionized water ultrasonically for 3 times, and centrifuge to get the adsorbed on the attapulgite Anthocyanin precipitation on the soil-loaded chitosan cross-linked cyclodextrin complex; add 80% ethanol to the anthocyanin precipitation for u...

Embodiment approach 2

[0039] This embodiment provides a method for extracting anthocyanins from mulberries. Take fresh mulberry fruits, remove the stems, beat them into a homogenate with a mixer, take 10 g of the homogenate and use the deep eutectic solvent choline chloride-acetic acid (molar ratio 2: 3, the water content is 15%wt) as the extraction solvent, and the solid-to-liquid ratio is 1g: 10mL, the ultrasonic power is 300W, the electrostatic field strength is 5kV, and the extraction temperature is 50°C, and the anthocyanin extract is obtained for 20min; the above anthocyanin extract Add 3 g of attapulgite-loaded chitosan cross-linked cyclodextrin complex, shake it on a shaking table at 30°C and 100 r / min for 5 hours, centrifuge to get the precipitate, wash it with deionized water ultrasonically for 3 times, and centrifuge to get the adsorbed on the attapulgite Anthocyanin precipitation on the soil-loaded chitosan cross-linked cyclodextrin complex; add 60% ethanol to the anthocyanin precipitati...

Embodiment approach 3

[0042] This embodiment provides a method for extracting anthocyanins in mulberries. Take fresh mulberry fruits, remove the stems, beat them into a homogenate with a mixer, take 10 g of the homogenate and use the deep eutectic solvent choline chloride-lactic acid-acetic acid (molar ratio 1:2:1, water content 30%wt) as the extraction solvent, under the conditions of solid-liquid ratio 1g:20mL, ultrasonic power 400W, electrostatic field strength 9kV, extraction temperature 60°C for 30min to obtain anthocyanin extract; Add 2 g of attapulgite-loaded chitosan cross-linked cyclodextrin complex to the obtained anthocyanin extract, shake it on a shaking table at 30°C and 100 r / min for 5 hours, centrifuge to collect the precipitate, and wash it with deionized water ultrasonically for 3 times. Centrifuge to obtain the anthocyanin precipitate adsorbed on the attapulgite-loaded chitosan cross-linked cyclodextrin complex; add ethanol with a mass fraction of 70% to the anthocyanin precipitate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
concentrationaaaaaaaaaa
Login to view more

Abstract

The invention relates to the field of edible pigments, and discloses a method for extracting plant anthocyanins. The plant containing anthocyanins is crushed and then added into a deep eutectic solvent; the anthocyanin extract is extracted by an electrostatic field ultrasonic extraction method; Add the attapulgite-loaded chitosan cross-linked cyclodextrin complex to obtain the precipitation of the anthocyanin complex adsorbed on the attapulgite-loaded chitosan cross-linked cyclodextrin complex; dissolve and desorb the anthocyanin complex precipitate in an organic solvent , centrifuge to obtain anthocyanin supernatant containing organic solvent; pass anthocyanin supernatant through microporous membrane to obtain purified anthocyanin solution containing organic solvent; remove organic solvent in anthocyanin solution and remove excess water to obtain target The product anthocyanins. The invention uses a deep eutectic solvent as an extraction solvent, which is green, environmentally friendly, and has a high extraction rate; the attapulgite-loaded chitosan cross-linked cyclodextrin compound is used to purify and separate anthocyanins, and the process is simple and the purification and separation efficiency is high.

Description

technical field [0001] The invention relates to the technical field of edible anthocyanins extraction, in particular to a method for extracting plant red / purple pigments. Background technique [0002] Anthocyanin (Anthocyanin) is a kind of natural pigment widely present in the cell fluid of plant roots, stems, leaves, fruits and other organs. flavonoid polyphenols. As a natural pigment, anthocyanins are safe, non-toxic, rich in resources, and have many health care effects on people, such as scavenging free radicals in the body, scavenging DPPH, anti-inflammatory, anti-cancer, delaying aging, preventing diabetes, and protecting eyesight. Therefore, anthocyanins are now widely used in medical, beauty, food and other industries. [0003] Anthocyanins, which constitute natural plant pigments, are often composed of multiple complexes with different polarity and physical and chemical properties, so it is difficult to prepare them in one step with a single solvent in the traditio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08C07H17/065C09B61/00B01J20/24B01J20/30
CPCB01J20/24C07H1/08C07H17/065C09B61/00
Inventor 王朝宇丁成新张文静毕艳红杨荣玲
Owner 黑龙江天阔源饮品有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap