GC-MS (Gas Chromatography-Mass Spectrometer) combined technology-based metabonomics research method for resistance of asarum and red peony root composition to cerebral ischemia-reperfusion injury

Abstract

The invention provides a GC-MS (Gas Chromatography-Mass Spectrometer) combined technology-based metabonomics research method for resistance of an asarum and red peony root composition to a cerebral ischemia-reperfusion injury. The method is realized through the following steps: (1) detecting and analyzing a serum sample and a urine sample of a mouse by adopting a GC-MS combined technology; (2) analyzing data by using PCA (Primary Component Analysis) and PLS-DA (Partial Least Square)-DA (Discriminant Analysis),and performing metabolite identification in combination with SPSS17.0 by using an NIST (National Institute of Standards and Technology) database to find out a final differential metabolite; (3) performing pathway analysis on the differential metabolite through MetPA online analysis software. According to the method, by analysis of the serum sample and the urine sample of the mouse through the GC-MS combined technology, respective identification of the differential metabolites of the serum sample and the urine sample of the mouse, and metabolic pathway analysis of the differential metabolites, the action mechanism of the asarum and red peony root composition resisting the cerebral ischemia-reperfusion injury can be comprehensively evaluated from the overall level, so that a demonstrative research is provided for explanation of the metabonomics and the action mechanism of a traditional Chinese medicine composition.

Description

technical field [0001] The invention belongs to the field of Chinese materia medica research, and in particular relates to a metabolomics research method of Xinshao prescription against cerebral ischemia-reperfusion injury based on GC-MS combined technology. technical background [0002] The effectiveness and practicability of traditional Chinese medicine are beyond doubt, but the research on traditional Chinese medicine prescriptions has not made breakthroughs so far. The two main problems are nothing more than the ingredients of traditional Chinese medicine prescriptions and the unclear mechanism of action. The complexity of the ingredients of traditional Chinese medicine is mainly due to the variety of its own compounds. Different compounds interact and change each other under different conditions, so it is difficult to measure their true ingredients. Its mechanism of action is mainly manifested in the synergistic integration of multiple components and targets, and it is ...

AI Technical Summary

Problems solved by technology

Although it has a good curative effect, its mechanism of action is still unclear, which limits the in-depth development of Xinshao prescription

Theoretically, the mechanism of action of Xinshao Recipe against cerebral ischemia-reperfusion injury should be "multi-channel, multi-component, multi-target", but there is still a lack of effective experimental methods and technical means to verify it.

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