Method of rapidly detecting accumulation of tumor tissue 2-HG
A tumor tissue, 2-HG technology, applied in the biological field, can solve the problems of atypia, increase patient burden and surgical risk, and take a long time to achieve the effect of fast detection time, rapid differential diagnosis, and reduction of patient risk.
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[0022] The isolated glioma brain tissue obtained during glioma surgery was rapidly detected, and 25-30 μg of the tumor tissue sample was excised and placed in a 1.5ml centrifuge tube; 100 μl of pre-cooled pure Methanol; Grind with a hand-held homogenizer for 1 min, and let stand for a while; Centrifuge at 14,000 rpm for 10 min; Take 40 μl of the supernatant, transfer it to a V-bottom GC special glass bottle, and fully dry it in a vacuum drying centrifuge, which takes about 25 min;. In a fume hood Add 40 μl of derivatization reagent MTBSTFA, tightly cap the bottle, and place it at 70°C for 25 minutes; let it cool naturally at room temperature, and put it on the machine, Agilent 7890A-5975C; the chromatographic column is HP-5MS, and the carrier gas is high-purity helium. 2-HG was identified by a combination of NIST database search and standard verification.
[0023] The test results show that using the detection method of the present invention to detect whether there is a high-l...
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