Method for widening detection range of immune colloidal gold

Abstract

The invention provides a method for widening a detection range of immune colloidal gold. According to the method, signal values GOD0.5 and GOD value of a reaction line (line T) of a colloidal gold test strip are determined by an immunochromatographic interpretation recorder at time t0.5 and time t, t represents time for complete reaction, t0.5 represents time of half of the signal value of complete reaction, when the quotient obtained by dividing GOD by GOD0.5 is larger than 1.5, the condition that no hook effect is produced is determined, a signal value of a detection result is subjected to concentration value calculation according to a linear regression equation, when the quotient obtained by dividing GOD by GOD0.5 is smaller than 1.5, the condition that the hook effect is produced is determined, and the signal value of the detection result is subjected to concentration value calculation according to a logistic curve-fitting four-parameter equation. With the adoption of the method, whether the hook effect exists in detection samples can be judged, and the sample with the hook effect can be directly intercepted and detected, reagents are not wasted, the detection result can be determined only by one-time detection, the detection is fast and easy, and the accuracy is high.

Description

technical field [0001] The invention relates to the technical field of in vitro immune detection, in particular to a method for expanding the detection range of immune colloidal gold. Background technique [0002] Colloidal gold is polymerized into gold particles of a specific size by chloroauric acid under the action of reducing agents such as white phosphorus, ascorbic acid, sodium citrate, tannic acid, etc., and becomes a stable colloidal state due to electrostatic interaction, called colloidal gold . Colloidal gold is negatively charged in a weak alkaline environment and can form a firm bond with the positively charged groups of protein molecules. Since this bond is electrostatic, it does not affect the biological characteristics of the protein. Immunocolloidal gold technology is a new type of immunolabeling technology that uses colloidal gold as a tracer marker to apply to antigens and antibodies. [0003] In the immunocolloidal gold quantitative test, the detection r...

AI Technical Summary

Problems solved by technology

[0005] The dilution method can eliminate the hook effect in the detection of immunocolloidal gold, but it cannot judge whether there is a hook effect in the test sample through one test. At least two tests are required, the operation is complicated, and the number of tests increases, resulting in the detection of reagents. It is a great waste and increases the operating error of the test personnel, which brings hidden dangers to the accuracy of the test results. At the same time, the accuracy of the test results of the diluted samples needs to be verified

Although the patent CN105044332A can judge whether there is a hook effect in the test sample through one test, at least two tests are required for the sample with the hook effect. The operation is complicated and the number of tests increases, resulting in a great waste of test reagents and It increases the operator's operating error, which brings hidden dangers to the accuracy of the test results. At the same time, the accuracy of the test results of the diluted samples needs to be verified.

Images