Method for widening detection range of immune colloidal gold

A detection range, colloidal gold technology, applied in measurement devices, biological tests, material inspection products, etc., can solve problems such as hidden dangers of detection results accuracy, waste of detection reagents, waste of detection reagents, and operational errors of detection personnel.

Inactive Publication Date: 2017-12-01
天津中新科炬生物制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The dilution method can eliminate the hook effect in the detection of immunocolloidal gold, but it cannot judge whether there is a hook effect in the test sample through one test. At least two tests are required, the operation is complicated, and the number of tests increases, resulting in the detection of reagents. It is a great waste and increases the operating error of the test personnel, which brings hidden dangers to the accuracy of the test results. At the same time, the accuracy of the test results of the diluted samples needs to be verified
Although the patent CN105044332A can judge whether there is a hook effect in the test sample through one test, at least two tests are required for the sample with the hook effect. The operation is complicated and the number of tests increases, resulting in a great waste of test reagents and It increases the operator's operating error, which brings hidden dangers to the accuracy of the test results. At the same time, the accuracy of the test results of the diluted samples needs to be verified.

Method used

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  • Method for widening detection range of immune colloidal gold
  • Method for widening detection range of immune colloidal gold
  • Method for widening detection range of immune colloidal gold

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0025] At present, the quantitative detection range of commonly used HCG immunocolloidal gold test strips is 10-15000mIu / mL at the most, and the highest of this method can only detect 15000mIu / mL, and the application of the method of the present invention can expand its quantitative detection range to 10-1600000mIu / mL, including the detection area without hook effect 10-25000mIu / mL and the area with barbs 25000-1600000mIu / mL, which avoids the hook shape produced by some abnormal pregnancy (high concentration HCG value) such as hydatidiform mole The false negative results brought about by the effect will cause misjudgment of the test results.

[0026] 1. t and t of HCG 0.5 determination of

[0027] Take HCG target standard samples with a low concentration of 5000mIu / mL and a high concentration of 10000mIu / mL, and use the immunochromatographic interpretation recorder to continuously detect the relationship between the signal value and time of the reaction line of the HCG immun...

Embodiment 2

[0036] 1. t and t of FOB 0.5 determination of

[0037] Take FOB target standard samples with low concentration of 0.2ug / mL and high concentration of 1ug / mL, and use the immunochromatographic interpretation recorder to continuously detect the relationship between the signal value and time of the reaction line of the FOB immunocolloidal gold test strip, see figure 2 As shown, the start time when the signal value of the reaction line is constant is the time of complete reaction, from figure 1 It can be seen from the curve that the t of high and low concentrations of FOB is 10 minutes, and the time for half of the signal value when the reaction line is completely reacted is t 0.5 ,From figure 1 It can be seen from the curve that the t of FOB with high and low concentration 0.5 for 140s.

[0038] 2. The method of judging whether the hook effect occurs in the FOB sample and accurately determining its concentration value

[0039] Can determine that the t of this FOB immune coll...

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Abstract

The invention provides a method for widening a detection range of immune colloidal gold. According to the method, signal values GOD0.5 and GOD value of a reaction line (line T) of a colloidal gold test strip are determined by an immunochromatographic interpretation recorder at time t0.5 and time t, t represents time for complete reaction, t0.5 represents time of half of the signal value of complete reaction, when the quotient obtained by dividing GOD by GOD0.5 is larger than 1.5, the condition that no hook effect is produced is determined, a signal value of a detection result is subjected to concentration value calculation according to a linear regression equation, when the quotient obtained by dividing GOD by GOD0.5 is smaller than 1.5, the condition that the hook effect is produced is determined, and the signal value of the detection result is subjected to concentration value calculation according to a logistic curve-fitting four-parameter equation. With the adoption of the method, whether the hook effect exists in detection samples can be judged, and the sample with the hook effect can be directly intercepted and detected, reagents are not wasted, the detection result can be determined only by one-time detection, the detection is fast and easy, and the accuracy is high.

Description

technical field [0001] The invention relates to the technical field of in vitro immune detection, in particular to a method for expanding the detection range of immune colloidal gold. Background technique [0002] Colloidal gold is polymerized into gold particles of a specific size by chloroauric acid under the action of reducing agents such as white phosphorus, ascorbic acid, sodium citrate, tannic acid, etc., and becomes a stable colloidal state due to electrostatic interaction, called colloidal gold . Colloidal gold is negatively charged in a weak alkaline environment and can form a firm bond with the positively charged groups of protein molecules. Since this bond is electrostatic, it does not affect the biological characteristics of the protein. Immunocolloidal gold technology is a new type of immunolabeling technology that uses colloidal gold as a tracer marker to apply to antigens and antibodies. [0003] In the immunocolloidal gold quantitative test, the detection r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76G01N33/558
CPCG01N33/76G01N33/558
Inventor 陈逸桐周洪锐李昀地杨延瑞宋兆瑞魏华英李洲
Owner 天津中新科炬生物制药股份有限公司
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