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Spirulina breeding method

A breeding method, the technology of spirulina, applied in the direction of bacteria, etc., can solve the problems of delayed and slow cultivation of spirulina, and achieve the effects of low energy consumption, promoting heat generation, and conducive to reproduction

Inactive Publication Date: 2017-12-08
DONGTAI CITY SPIRULINA BIO ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the cultivation method of spirulina is generally to cultivate spirulina by sprinkling the algae seeds into the culture medium, and then cultivating for a few days after controlling the temperature conditions to obtain spirulina, but this method is slow in the cultivation process, During the research process, it was found that Spirulina can reproduce by absorbing carbon dioxide and nitrogen compounds, and the speed of reproduction is relatively fast, and the speed of reproduction with carbon dioxide and nitrogen compounds under low light conditions has achieved amazing results, but through external The weak light control can not extend into the medium, and the algae at the bottom of the medium cannot feel the low light environment, resulting in the delay of Spirulina cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A culture method for spirulina, comprising steps as follows:

[0021] a. Pre-disinfection: perform high-temperature disinfection on the closed reactor; the temperature for high-temperature disinfection is 220°C; while heating, it is cleaned by sodium hypochlorite, and the heating time is controlled at 11 minutes.

[0022] b. Preparation of culture medium: prepare culture medium in a closed reactor, and control the temperature in the reactor to 23°C; the culture medium includes the following components by weight: 100 parts of water, 30 parts of calcium hydroxide, 10 parts of malic acid, bran 10 parts of skin, 20 parts of soybean meal, 10 parts of aluminum nitride, 10 parts of calcium carbide, 5 parts of hydrochloric acid; the concentration of the described malic acid is 4 mol / L; the concentration of the described hydrochloric acid is 2 mol / L; The hot gas is controlled at a temperature ranging from 23°C.

[0023] c. Connect fluorescent sticks: Put multiple fluorescent st...

Embodiment 2

[0027] A culture method for spirulina, comprising steps as follows:

[0028] a. Pre-disinfection: high-temperature disinfection of the closed reactor; the temperature of high-temperature disinfection is 250°C; while heating, it is cleaned by sodium hypochlorite, and the heating time is controlled at 13 minutes.

[0029] b. Preparation of culture medium: prepare culture medium in a closed reactor, and control the temperature in the reactor to 24°C; the culture medium includes the following components by weight: 100 parts of water, 35 parts of calcium hydroxide, 16 parts of malic acid, bran 17 parts of skin, 25 parts of soybean meal, 15 parts of aluminum nitride, 16 parts of calcium carbide, and 8 parts of hydrochloric acid; the concentration of described malic acid is 4.5mol / L; the concentration of described hydrochloric acid is 2.5mol / L; The hot gas is controlled at a temperature ranging from 24°C.

[0030] c. Connect fluorescent sticks: Put multiple fluorescent sticks into t...

Embodiment 3

[0034] A culture method for spirulina, comprising steps as follows:

[0035] a. Pre-disinfection: high-temperature disinfection of the closed reactor; the temperature of high-temperature disinfection is 295°C; while heating, it is cleaned by sodium hypochlorite, and the heating time is controlled at 15 minutes.

[0036] b. Preparation of culture medium: prepare culture medium in a closed reactor, and control the temperature in the reactor to 24°C; the culture medium includes the following components by weight: 100 parts of water, 40 parts of calcium hydroxide, 20 parts of malic acid, bran 20 parts of skins, 30 parts of soybean meal, 20 parts of aluminum nitride, 20 parts of calcium carbide, 10 parts of hydrochloric acid; the concentration of said malic acid is 5mol / L; the concentration of said hydrochloric acid is 3mol / L; Control the temperature in the range of 24°C.

[0037] c. Connect fluorescent sticks: Put multiple fluorescent sticks into the closed reactor, and put the f...

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Abstract

The invention discloses a spirulina breeding method, which comprises the steps of pre-disinfection, culture medium preparation, putting in of fluorescent sticks, inoculation and introduction of carbon dioxide; according to the spirulina breeding method, the multiple fluorescent sticks are put into an airtight reactor in a nannochloropsis oculata culture process, by putting the fluorescent sticks evenly into the reactor, a low light environment for spirulina species filament cultivation is created, through the fluorescent sticks, the energy consumption is low, and weak light can penetrate to the bottom of a culture medium, so algae species in the culture medium can obtain fully weak light, enabling the cultivation environment of the algae species to be qualitatively changed. The culture medium disclosed by the invention is more improved than the traditional medium. The addition of malic acid and calcium hydroxide in the culture medium can generate neutralization reaction, promote the generation of heat, and facilitate the reproduction of spirulina.

Description

technical field [0001] The invention relates to a cultivation method of spirulina. Background technique [0002] At present, the cultivation method of spirulina is generally to cultivate spirulina by sprinkling the algae seeds into the culture medium, and then cultivating for a few days after controlling the temperature conditions to obtain spirulina, but this method is slow in the cultivation process, During the research process, it was found that Spirulina can reproduce by absorbing carbon dioxide and nitrogen compounds, and the speed of reproduction is relatively fast, and the speed of reproduction with carbon dioxide and nitrogen compounds under low light conditions has achieved amazing results, but through external The low-light control can not extend into the medium, and the algae at the bottom of the medium cannot feel the low-light environment, resulting in the delay of Spirulina cultivation. Contents of the invention [0003] Aiming at the deficiencies of the abo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 高志刚魏新林汪世军刘晓燕王俊巍吴援援陈景惠王海宇
Owner DONGTAI CITY SPIRULINA BIO ENG CO LTD
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