Micromolecule for activating LRIG1 expression in brain glioma U251 cells and screening method and application of micromolecule
A technology for brain glioma and screening methods, applied in the direction of DNA/RNA fragments, organic active ingredients, recombinant DNA technology, etc.
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[0022] dsRNA Design dsLRIG1-712 is a dsRNA molecule with a length of 21 nucleotide base pairs designed for the relative transcription start point -712 of the LRIG1 gene promoter region and complementary to the promoter. The control dsRNA (dsControl) is non-homologous to the known human genome sequence, and is a dsRNA sequence with the same length of 21 nucleotide base pairs.
[0023] Use the Genebank database to find the sequence of the promoter region of the LRIG1 gene, and use the -712 sequence upstream of the promoter as a template for designing dsRNA. The specific sequence is: dsLRIG1-712: Sense: UUU CCC ACC CAA GGU GUG A[dT][dT]; Antisense: UCA CAC CUU GGG UGG GAA A[dT][dT].
[0024] Cell culture and transfection U251 cells were grown in DMEM medium containing 10% fetal bovine serum at 37°C, 5% CO 2 Incubator cultivation. The experiment was divided into three groups: blank group, control group (transfected with dsControl) and experimental group (transfected with candida...
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