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Stem cell secretin for resisting aging as well as preparation method and application of stem cell secretin

A secretin and stem cell technology, applied in the field of biomedicine, can solve the problems of bacterial contamination and tumorigenicity, and achieve the effect of delaying aging

Inactive Publication Date: 2017-12-08
深圳中旭细胞再生医学研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There are still certain risks in clinical application of stem cells, such as bacterial contamination and certain tumorigenicity

Method used

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  • Stem cell secretin for resisting aging as well as preparation method and application of stem cell secretin

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Embodiment 1

[0023] In the embodiment of the present invention, the preparation of stem cell secretin: using human fetal-derived MSCs (bone marrow mesenchymal stem cells, mesenchymal stem cells) (FMSCs), cultured for 3 days after identification by flow cytometry, collected cultured cells, buffered with PBS After washing twice with liquid, water was added to obtain a lysate. After centrifugation at 4°C and 10,000 r / min for 30 minutes at low temperature, the supernatant was collected, and 1.5% human serum albumin was added to obtain stem cell secretin.

Embodiment 2

[0025] In the embodiment of the present invention, the preparation of stem cell secretin: using human fetal-derived MSCs (bone marrow mesenchymal stem cells, mesenchymal stem cells) (FMSCs), cultured for 2 days after identification by flow cytometry, collected cultured cells, buffered with PBS After washing twice with liquid, water was added to obtain a lysate. After centrifugation at 1° C. and 10,000 r / min for 40 minutes at low temperature, the supernatant was collected, and 1% human serum albumin was added to obtain stem cell secretin.

Embodiment 3

[0027] In the embodiment of the present invention, the preparation of stem cell secretin: using human fetal-derived MSCs (bone marrow mesenchymal stem cells, mesenchymal stem cells) (FMSCs), cultured for 5 days after identification by flow cytometry, collected cultured cells, buffered with PBS After washing twice with liquid, water was added to obtain a lysate. After centrifugation at 5° C. and 14000 r / min for 20 minutes at low temperature, the supernatant was collected, and 2% human serum albumin was added to obtain stem cell secretin.

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Abstract

The invention discloses a stem cell secretin for anti-aging and its preparation method and application. During the preparation, mesenchymal stem cells are collected, cultured for 2-5 days, the cultured cells are collected, washed with PBS buffer solution, and then added with water to lyse The lysate is obtained; the lysate is then centrifuged at low temperature, the supernatant is collected, and human albumin with a mass of 1-2% of the supernatant is added to obtain the stem cell secretin. Mesenchymal stem cells include bone marrow, umbilical cord, placenta and fat. The stem cell secretin prepared by the invention can have an anti-aging effect, and the stem cell secretin is derived from bone marrow mesenchymal stem cells. The present invention proves that the secretin, compared with the untreated one, can delay the senescence of human adult MSCs, so it has the potential of delaying senescence. The medicine of the present invention can be administered intravenously or locally.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a stem cell secretin for anti-aging and its preparation method and application. Background technique [0002] Stem cells are a kind of pluripotent cells with self-renewing ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells (ES cells) and adult stem cells (somatic stem cells) according to their developmental stages. Stem cells are divided into three categories according to their developmental potential: totipotent stem cells (TSCs), pluripotent stem cells (pluripotent stem cells), and unipotent stem cells (unipotent stem cells). Stem cells are not fully differentiated and immature cells, which have the potential to regenerate various tissues and organs and the human body. They are called "universal cells" in the medical field. [0003] Stem cells are known as the "fountain of youth" and have gre...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12N5/0775A61K35/28A61P39/06
CPCC12P21/00A61K35/28C12N5/0662C12N5/0663
Inventor 张锦芳卓成柳吴中泽
Owner 深圳中旭细胞再生医学研究有限公司
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