Method for detecting influence of electronic cigarette product to peroxidating of cell lipid

A lipid peroxidation and electronic cigarette technology, which is applied in biochemical equipment and methods, measuring devices, and microbial determination/inspection, etc., can solve problems such as time-consuming and labor-intensive, and no unified standard method.

Inactive Publication Date: 2017-12-12
CHINA TOBACCO YUNNAN IND
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Problems solved by technology

However, there are a large number of primary formulas, and sensory evaluation of all of them is time-consuming and labor-intensive, and the sensory evaluation focuses on screening the style and taste of the product. How to use biolog

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  • Method for detecting influence of electronic cigarette product to peroxidating of cell lipid

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Embodiment Construction

[0045] The present invention will be described in detail below in conjunction with specific examples, but the present invention is not limited.

[0046] This example is aimed at testing the effects of four foreign commercially available e-cigarette liquids on lipid peroxidation of human lung fibroblasts. The specific steps are as follows:

[0047] (1) Pretreatment of the test substance: absorb a certain amount of e-cigarette stock solution, mix and dilute it according to the volume ratio of the e-cigarette stock solution and serum-free cell culture medium at a ratio of 1:5, place it for 24 hours, and filter it with a sterile filter Sterilize to obtain the solution to be tested, and store it at -80°C until use;

[0048](2) Preparation of single-cell suspension of human lung fibroblasts: After resuscitating human lung fibroblasts, inoculate them into cell culture flasks and place them at 37°C and 5vt% CO 2 Cultivate in an incubator, observe the confluence and morphology of the ...

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Abstract

The invention discloses a method for detecting influence of an electronic cigarette product to peroxidating of cell lipid. The method comprises the following steps: pretreatment of test samples, preparation of human lung fibroblasts single-cell suspension, calculating of concentration of cells of the human lung fibroblasts single-cell suspension, cell inoculation of the human lung fibroblasts single-cell suspension, grouping of the test samples, setting of detection dosage of the test samples, preparation of cultured products of the test samples, incubation of the test samples, collection of incubated products of the test samples, cracking and centrifuging of the incubated products of the test samples, calculation of the content of malonyldialdehyde and the like. By effective treatment to samples, optimal setting on the detecting dosage and correct selection of target cells, the method can accurately and effectively detect the influence of the electronic cigarette product to peroxidating of the cell lipid.

Description

technical field [0001] The invention belongs to the technical field of biological effect evaluation of tobacco products, in particular to a method for detecting the influence of electronic cigarette products on cell lipid peroxidation. Background technique [0002] As people pay more and more attention to health, higher requirements are put forward for tobacco products, not only to have a better taste, but also to reduce the adverse feelings of the human body as much as possible. E-cigarette products avoid the harm caused by the complex mixture produced by the combustion of traditional cigarette products. At this stage, it has become one of the new directions for foreign tobacco companies to shift from traditional cigarette products. During the use of electronic cigarette products, the liquid composed of nicotine, flavoring substances, etc. is converted into a mixture similar to cigarette smoke by electric heating or electronic atomization, and then the atomized glycerol / nic...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/5044G01N33/5038
Inventor 管莹夭建华李雪梅熊国行米其利高茜朱洲海
Owner CHINA TOBACCO YUNNAN IND
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