Method for recycling waste light disk and method for culturing PC12 cells by virtue of recycled light disk
A technology of discarded optical discs and PC12, which is applied to animal cells, nervous system cells, vertebrate cells, etc., and can solve problems such as lengthy procedures, environmental sensitivity, difficulty in purification, and high manufacturing costs
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[0044] The present invention will be described in detail below in conjunction with the accompanying drawings.
[0045] 2. Experimental process
[0046] 2.1 Characterization of waste discs. The scrap DVD media itself consists mainly of five layers, including a label layer, two PC layers, a reflective layer and a dye data layer (Scheme 1a). The surface of the two PC layers has a prefabricated spiral groove pattern. Add the dye data layer on top of the groove and bottom PC layer. A writing laser changes the dye data layer by leaving spots in the trenches, which represent a digital code for storing information. A reflective layer is added that reflects the reading laser light back to the detector after reading the spots in the dye data layer. Finally, silkscreen the label layer onto the top. In order to facilitate the study of cell behavior on discarded DVD substrates, the applicant used a utility knife to carefully peel off the label layer and the top transparent PC layer (S...
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