Method for recycling waste light disk and method for culturing PC12 cells by virtue of recycled light disk

A technology of discarded optical discs and PC12, which is applied to animal cells, nervous system cells, vertebrate cells, etc., and can solve problems such as lengthy procedures, environmental sensitivity, difficulty in purification, and high manufacturing costs

Inactive Publication Date: 2017-12-19
HUNAN NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

Although these materials have been valued due to their biocompatibility, tunable properties and low-risk immunogenicity, natural materials, as well as most existing man-made materials, suffer from several disadvantages: natural materials including their complex struc

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  • Method for recycling waste light disk and method for culturing PC12 cells by virtue of recycled light disk
  • Method for recycling waste light disk and method for culturing PC12 cells by virtue of recycled light disk
  • Method for recycling waste light disk and method for culturing PC12 cells by virtue of recycled light disk

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Embodiment Construction

[0044] The present invention will be described in detail below in conjunction with the accompanying drawings.

[0045] 2. Experimental process

[0046] 2.1 Characterization of waste discs. The scrap DVD media itself consists mainly of five layers, including a label layer, two PC layers, a reflective layer and a dye data layer (Scheme 1a). The surface of the two PC layers has a prefabricated spiral groove pattern. Add the dye data layer on top of the groove and bottom PC layer. A writing laser changes the dye data layer by leaving spots in the trenches, which represent a digital code for storing information. A reflective layer is added that reflects the reading laser light back to the detector after reading the spots in the dye data layer. Finally, silkscreen the label layer onto the top. In order to facilitate the study of cell behavior on discarded DVD substrates, the applicant used a utility knife to carefully peel off the label layer and the top transparent PC layer (S...

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Abstract

The invention discloses a method for recycling waste light disk and a method for culturing PC12 cells by virtue of the recycled light disk. According to the methods, electronic waste is directly utilized and is applied to nervous tissue engineering, so that ready-made waste materials with various micron/nanometer structures can be provided so as to solve problems in the supplying and manufacturing of micron/nanometer patterns, and a new hopeful environmental protection manner can be provided; the application proves that wasted electronic products can be directly and repeated used as highly anisotropic substrates in the in vitro study of cell behaviors; PC12 cells cultured on the two substrates with different morphologies present different cell morphologies; and more chances are provided for the combination of theories and advantages of the information technique field and other fields, and a novel biological interface is hopefully constructed and designed by combining methods of biochemical building blocks, projects, materials and the like and is applied to the fields of biomedicines, biosensors, energy and the like.

Description

technical field [0001] The invention relates to CD recycling technology and nerve tissue engineering technology, in particular to a recycling method for discarded CDs and a method for cultivating PC12 cells by utilizing the recovered CDs. Background technique [0002] Due to the rapid changes in information technology and the increasingly fierce competitive environment, various electronic consumer goods manufactured using various micro-nano manufacturing methods are discarded as electronic waste, which is the fastest growing pollution problem in the world. Since the 1960s, Moore's Law has continuously driven the information technology revolution and driven the innovative development of today's increasingly ubiquitous personal computers, high-speed Internet and smartphones. With the increasing demand for better performance in the fields of data storage, digital display, Internet of Things and artificial intelligence, such as storage devices (optical and magnetic storage media...

Claims

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Application Information

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IPC IPC(8): C12N5/079
CPCC12N5/0618
Inventor 黄伟涛路娇扬张新星朱秋燕张福瑞丁学知夏立秋罗红群李念兵
Owner HUNAN NORMAL UNIVERSITY
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