Use of waste fish scales in preparing anisotropic substrate

Abstract

The present application uses waste fish scales as an anisotropic substrate to simulate ECM for directional regulation and control of cancer cells and drug sensitivity evaluation. A result shows that an outer surface covering area comprises a groove-shaped structure and an inner surface is of a tightly-arranged ordered fiber structure; main components of the outer and inner surfaces are collagen and hydroxyapatite, the outer surface contains more hydroxyapatite and less collagen, while the inner surface contains less hydroxyapatite and more collagen; and the outer and inner surfaces have relatively strong cell adhesion. The fish scales have an effect of inhibiting tumor cell proliferation and are free of cytotoxicity. An orienting capability of oriented cells from high to low is as follows in sequence: an undamaged inner surface> a covering area> a damaged inner surface> an exposing area. The waste fish scales have advantages of a wide material source, waste recycling, no patterning and the like, facilitate cell directional regulation and control, eliminate differences of in-vivo and in-vitro research results, deepen objective law understanding on tumor occurrence and development, and provide a reliable platform for more accurate and effective in-vitro drug screening.

Description

technical field [0001] The invention relates to biotechnology, in particular to the application of waste fish scales in the preparation of anisotropic substrates. Background technique [0002] There are various micro / nano features in the natural microenvironment (such as Extracellular matrix, ECM) on which cells depend, and the migration, proliferation, differentiation, tissue development and metabolism of cells are all affected by them. The ECM usually self-assembles into a nano- to micro-scale three-dimensional network structure, which provides crucial chemical and physical cues for the triggering and regulation of cell behavior, and can also change the drug sensitivity of cells5. Traditional 2D culture materials lack similar biophysical and chemical cues (such as micro / nanopatterns, stiffness, and chemical composition) of the in vivo physiological environment, and cannot truly reflect the in vivo conditions, resulting in significantly different results of in vivo and in v...

AI Technical Summary

Problems solved by technology

However, these studies have overlooked the great natural advantages of the natural structure of fish scales in modulating cell behavior, especially tumor cell behavior, and mimicking the natural ECM.

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